Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats
To investigate whether time-restricted feeding (TRF) can alleviate cardiac remodeling in spontaneously hypertensive rats (SHRs) by regulating autophagy levels. A 16-week TRF intervention was conducted on Wistar Kyoto (WKY) rats and SHRs, with dietary intake confined to the interval from 9:00 am to 5...
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| Format: | Article |
| Language: | English |
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Nature Research
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120427/ http://psasir.upm.edu.my/id/eprint/120427/1/120427.pdf |
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| author | Yi, Xin Abas, Razif Raja Muhammad Rooshdi, Raja Abdul Wafy Yan, Jie Liu, Canzhang An, Jiaxu Daut, Ummi Nadira |
| author_facet | Yi, Xin Abas, Razif Raja Muhammad Rooshdi, Raja Abdul Wafy Yan, Jie Liu, Canzhang An, Jiaxu Daut, Ummi Nadira |
| author_sort | Yi, Xin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | To investigate whether time-restricted feeding (TRF) can alleviate cardiac remodeling in spontaneously hypertensive rats (SHRs) by regulating autophagy levels. A 16-week TRF intervention was conducted on Wistar Kyoto (WKY) rats and SHRs, with dietary intake confined to the interval from 9:00 am to 5:00 pm each day. The study examined the impact of TRF on blood pressure (BP), cardiac morphology and function, and the expression levels of key proteins involved in autophagy and its associated signaling cascades. Transmission Electron Microscopy (TEM) was utilized to further evaluate autophagic changes in left ventricular (LV) tissues. TRF significantly mitigated systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean blood pressure (MBP) in SHRs. Additionally, TRF improved ejection fraction (EF) and diminished interventricular septal thickness at end-diastole (IVS-d). The study further revealed that TRF enhanced the expression of microtubule-associated protein-I light chain 3 (LC3-I), while reducing that of microtubule-associated protein-II light chain 3 (LC3-II). Moreover, TRF suppressed the expression levels of Beclin-1, phosphorylated phosphoinositide 3-kinase (p-PI3K), phosphorylated protein kinase B (p-AKT), and phosphorylated mechanistic target of rapamycin (p-mTOR) in the LV tissues. TEM analysis confirmed that TRF could inhibit autophagy levels in the LV tissues. TRF can attenuate cardiac remodeling in SHRs by regulating autophagy levels. |
| first_indexed | 2025-11-15T14:48:22Z |
| format | Article |
| id | upm-120427 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:48:22Z |
| publishDate | 2025 |
| publisher | Nature Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1204272025-10-01T06:32:19Z http://psasir.upm.edu.my/id/eprint/120427/ Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats Yi, Xin Abas, Razif Raja Muhammad Rooshdi, Raja Abdul Wafy Yan, Jie Liu, Canzhang An, Jiaxu Daut, Ummi Nadira To investigate whether time-restricted feeding (TRF) can alleviate cardiac remodeling in spontaneously hypertensive rats (SHRs) by regulating autophagy levels. A 16-week TRF intervention was conducted on Wistar Kyoto (WKY) rats and SHRs, with dietary intake confined to the interval from 9:00 am to 5:00 pm each day. The study examined the impact of TRF on blood pressure (BP), cardiac morphology and function, and the expression levels of key proteins involved in autophagy and its associated signaling cascades. Transmission Electron Microscopy (TEM) was utilized to further evaluate autophagic changes in left ventricular (LV) tissues. TRF significantly mitigated systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean blood pressure (MBP) in SHRs. Additionally, TRF improved ejection fraction (EF) and diminished interventricular septal thickness at end-diastole (IVS-d). The study further revealed that TRF enhanced the expression of microtubule-associated protein-I light chain 3 (LC3-I), while reducing that of microtubule-associated protein-II light chain 3 (LC3-II). Moreover, TRF suppressed the expression levels of Beclin-1, phosphorylated phosphoinositide 3-kinase (p-PI3K), phosphorylated protein kinase B (p-AKT), and phosphorylated mechanistic target of rapamycin (p-mTOR) in the LV tissues. TEM analysis confirmed that TRF could inhibit autophagy levels in the LV tissues. TRF can attenuate cardiac remodeling in SHRs by regulating autophagy levels. Nature Research 2025-05-15 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/120427/1/120427.pdf Yi, Xin and Abas, Razif and Raja Muhammad Rooshdi, Raja Abdul Wafy and Yan, Jie and Liu, Canzhang and An, Jiaxu and Daut, Ummi Nadira (2025) Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats. Scientific Reports, 15 (1). art. no. 16973. pp. 1-15. ISSN 2045-2322 https://www.nature.com/articles/s41598-025-01587-x?error=cookies_not_supported&code=68be07d9-dfb4-4de0-ac1a-7bdc9b49304e 10.1038/s41598-025-01587-x |
| spellingShingle | Yi, Xin Abas, Razif Raja Muhammad Rooshdi, Raja Abdul Wafy Yan, Jie Liu, Canzhang An, Jiaxu Daut, Ummi Nadira Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats |
| title | Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats |
| title_full | Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats |
| title_fullStr | Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats |
| title_full_unstemmed | Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats |
| title_short | Time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats |
| title_sort | time-restricted feeding attenuated hypertension-induced cardiac remodeling by modulating autophagy levels in spontaneously hypertensive rats |
| url | http://psasir.upm.edu.my/id/eprint/120427/ http://psasir.upm.edu.my/id/eprint/120427/ http://psasir.upm.edu.my/id/eprint/120427/ http://psasir.upm.edu.my/id/eprint/120427/1/120427.pdf |