Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing

QR Code

Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing

Objective: Extraction of high-quality RNA is crucial for understanding the molecular dynamics of microbiomes in the growth and development of paddy plants. However, paddy soil poses challenges due to contaminants such as humic substances and its clayish nature, which lead to RNA adsorption and reduc...

Full description

Bibliographic Details
Main Authors:	Sivaprakasam, Sumitra, Tan, Yee Fan, Kumarasan, Yukgehnaish, Mohd Hata, Erneeza, Vadamalai, Ganesan, Petersen, Bent, Sicheritz-Pontén, Thomas, Parimannan, Sivachandran, Rajandas, Heera
Format:	Article
Language:	English
Published:	BioMed Central 2025
Online Access:	http://psasir.upm.edu.my/id/eprint/120152/ http://psasir.upm.edu.my/id/eprint/120152/1/120152.pdf

Similar Items

Complete genome sequence of potential plant growth-promoting Bacillus altitudinis strain AIMST-CREST03 isolated from paddy field bulk soil
by: Sivaprakasam, Sumitra, et al.
Published: (2024)

Complete genome sequence of plant growth-promoting Bacillus stratosphericus AIMST-CREST02 isolated from bulk soil of a paddy field
by: Sivaprakasam, Sumitra, et al.
Published: (2024)

Draft genome sequence of Bacillus tropicus strain UPM-CREST01, isolated from the bulk paddy soil at Kampung Gajah, Perak
by: Mohd Azim Khan, Nur Arisa, et al.
Published: (2022)

Gut microbiota metagenomics in aquaculture: factors influencing gut microbiome and its physiological role in fish
by: Yukgehnaish, Kumarasan, et al.
Published: (2020)

Advancement of metatranscriptomics towards productive agriculture and sustainable environment: a review
by: Sharuddin, Siti Suhailah, et al.
Published: (2022)

Functional diversity of active microbial mat communities inferred fromlipid biomarkers and metatranscriptomics
by: Campbell, Matthew Arthur
Published: (2020)

Characterization of the nearly complete mitochondrial genome of ochraceous darkies, Euphaea ochracea Selys, 1859 (Odonata: Zygoptera: Euphaeidae) and phylogenetic analysis
by: Marylin Miga, Marylin Miga, et al.
Published: (2023)

Characterization of the nearly complete mitochondrial genome of ochraceous darkies, Euphaea ochracea Selys, 1859 (Odonata: Zygoptera: Euphaeidae) and phylogenetic analysis
by: Marylin Miga, Marylin Miga, et al.
Published: (2023)

Characterization of the complete mitogenome data of Ischyja marapok (Lepidoptera: Noctuoidea: Erebidae) from Malaysia
by: Marylin Miga, Marylin Miga, et al.
Published: (2023)

Complete mitochondria! genome data and phylogenetic analysis of the Great Marquis, Bassarona dunya (Doubleday, 1848) (Lepidoptera: Nymphalidae: Limenitidinae) from Malaysia
by: Marylin Migaa, Marylin Migaa, et al.
Published: (2022)

Insights into bacterial community metatranscriptome and metabolome in river water influenced by palm oil mill effluent final discharge
by: Sharuddin, Siti S, et al.
Published: (2023)

A review of Pangasiid catfish genomics for conservation and aquaculture: current status and way forward
by: Abdul-Halim, Siti Amalia Aisyah, et al.
Published: (2024)

Actinomycetes as plant growth promoter of rice plants and biocontrol agent against bacterial leaf streak disease
by: Mohd Hata, Erneeza
Published: (2019)

Characterization of the nearly complete mitochondrial genome of ochraceous darkies, Euphaea ochracea Selys, 1859 (Odonata: Zygoptera: Euphaeidae) and phylogenetic analysis
by: Marylin Miga, Marylin Miga, et al.
Published: (2023)

Characterization of the nearly complete mitochondrial genome of ochraceous darkies, Euphaea ochracea Selys, 1859 (Odonata: Zygoptera: Euphaeidae) and phylogenetic analysis
by: Miga, Marylin, et al.
Published: (2023)

Characterization of the nearly complete mitochondrial genome of ochraceous darkies, Euphaea ochracea Selys, 1859 (Odonata: Zygoptera: Euphaeidae) and phylogenetic analysis
by: Miga, Marylin, et al.
Published: (2023)

Complete mitochondria! genome data and phylogenetic analysis of the Great Marquis, Bassarona dunya (Doubleday, 1848) (Lepidoptera: Nymphalidae: Limenitidinae) from Malaysia
by: Migaa, Marylin, et al.
Published: (2023)

Characterization of the nearly complete mitochondrial genome of ochraceous darkies, Euphaea ochracea Selys, 1859 (Odonata: Zygoptera: Euphaeidae) and phylogenetic analysis
by: Miga, Marylin, et al.
Published: (2023)

Transcriptomics-based analysis using RNA-Seq of the coconut (Cocos nucifera) leaf in response to yellow decline phytoplasma infection
by: Nejat, Naghmeh, et al.
Published: (2015)

In Vitro Production and Infectivity of Oncobasidium Theobromae Talbot and Keane Basidiospores
by: Vadamalai, Ganesan
Published: (1999)

Morphological, pathogenic and molecular characterization of Lasiodiplodia theobromae: a causal pathogen of black rot disease on kenaf seeds in Malaysia
by: M., Norhayati, et al.
Published: (2016)

An empirical model of the bulk shipping market
by: Lun, V., et al.
Published: (2009)

Complete mitochondria! genome data and phylogenetic analysis of the Great Marquis, Bassarona dunya (Doubleday, 1848) (Lepidoptera: Nymphalidae: Limenitidinae) from Malaysia
by: Migaa, Marylin, et al.
Published: (2023)

The efficacy of using concept of relatability in teaching of literature for Malaysian school students
by: Vasundera, Kumarasan
Published: (2024)

Failure prediction in bulk metal forming process
by: Topa, Ameen, et al.
Published: (2014)

Contamination in the bulk agri-commodity logistics chain
by: Jackson, Elizabeth
Published: (2020)

Vibration-induced phenomena in bulk granular materials
by: Golovanevskiy, Vladimir, et al.
Published: (2011)

Intrinsic vacancies in cubic-zirconia bulk and surface
by: Sun, P., et al.
Published: (2010)

Study of hysteresis and chaotic dynamics in bulk ferromagnet
by: Pek, Lan Toh, et al.
Published: (2009)

Phytoplasma detection in coconut palm and other tropical crops
by: Nejat, Naghmeh, et al.
Published: (2010)

The relationship between miRNA biogenesis and RNA splicing
by: Parsons, Aimee
Published: (2018)

Liquefaction risks on bulk cargoes carrying Bukit Goh bauxite in accordance International Maritime Solid Bulk Cargoes (IMSBC) code
by: Syazwan, Sukri
Published: (2017)

A comprehensive 3D dosimetric analysis of the vienna ring applicator in high-dose-rate (HDR) brachytherapy for cervical cancer treatment: TLD measurement
by: Sivaprakasam, Pugalya
Published: (2024)

Evaluation of patient dose during multi-catheter insertion in CT-guided interstitial liver brachytherapy
by: Sivaprakasam, Puviya
Published: (2024)

On the coexistence pressure between the bulk and adsorbed argon on substrates of different strength – Temperature dependence of the characteristics of the adsorbate
by: Prasetyo, L., et al.
Published: (2019)

Factors influencing job performance among employees in Bulk and Break Bulk Terminal Department Of Johor Port Berhad / Siti Afifah Zulkefle
by: Zulkefle, Siti Afifah
Published: (2017)

Liquefaction risks on bulk cargoes carrying unamended & amended Gebeng bauxite in accordance to International Maritime Solid Bulk Cargoes (IMSBC) code
by: Muhammad Fat-Hi Al Juwaini, Pahrol
Published: (2017)

Paddy and rice.
by: Shamsudin, Mad Nasir, et al.
Published: (2012)

Modeling the performance of bulk-carriers for Islamic equity investors
by: Abdullah, Adam, et al.
Published: (2017)

A Critical Investigation of the Bulk Shipping Commodities Trade
by: Fernandes, Rohit Walter
Published: (2010)