Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing

Objective: Extraction of high-quality RNA is crucial for understanding the molecular dynamics of microbiomes in the growth and development of paddy plants. However, paddy soil poses challenges due to contaminants such as humic substances and its clayish nature, which lead to RNA adsorption and reduc...

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Main Authors: Sivaprakasam, Sumitra, Tan, Yee Fan, Kumarasan, Yukgehnaish, Mohd Hata, Erneeza, Vadamalai, Ganesan, Petersen, Bent, Sicheritz-Pontén, Thomas, Parimannan, Sivachandran, Rajandas, Heera
Format: Article
Language:English
Published: BioMed Central 2025
Online Access:http://psasir.upm.edu.my/id/eprint/120152/
http://psasir.upm.edu.my/id/eprint/120152/1/120152.pdf