Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development
Ruxolitinib is a Janus kinase (JAK) inhibitor that inhibits the JAK/STAT signalling pathway by targeting JAK1 and JAK2, which are crucial for regulating astrogliogenesis. This study assessed the effect of ruxolitinib (5 and 30 mg/kg/day) on developing mouse brains by administering it to pregnant mic...
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| Format: | Article |
| Language: | English |
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Neurotak Publishing
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118733/ http://psasir.upm.edu.my/id/eprint/118733/1/118733.pdf |
| _version_ | 1848867585562509312 |
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| author | Abdullah, Amirah Nabilah Fasihah Zamri, Auni Alias, Khairunnisa Azhar, Nur Munawwarah Ling, King Hwa Cheah, Pike See Zainal Abidin, Shahidee |
| author_facet | Abdullah, Amirah Nabilah Fasihah Zamri, Auni Alias, Khairunnisa Azhar, Nur Munawwarah Ling, King Hwa Cheah, Pike See Zainal Abidin, Shahidee |
| author_sort | Abdullah, Amirah Nabilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Ruxolitinib is a Janus kinase (JAK) inhibitor that inhibits the JAK/STAT signalling pathway by targeting JAK1 and JAK2, which are crucial for regulating astrogliogenesis. This study assessed the effect of ruxolitinib (5 and 30 mg/kg/day) on developing mouse brains by administering it to pregnant mice from E7.5 to E20.5. No adverse effects were observed in the treated mice. The brains of P1.5 pups were collected, and RNA was extracted to assess markers of neurogenesis and astrogliogenesis through RT-qPCR. The results revealed a significant decrease in Gfap expression (p<0.0001) in both ruxolitinib-treated groups compared to the control, indicating a suppression of astrogliogenesis. Additionally, S100β expression (p<0.05) was significantly reduced in the 30 mg/kg ruxolitinib-treated group. In contrast, the expression of neuronal markers vGLuT1 (p<0.01) and vGLuT2 (p<0.01) increased significantly in the 30 mg/kg treated group, suggesting enhanced neuronal differentiation. Furthermore, 5 and 30 mg/kg ruxolitinib-treated groups showed a significant increase in GAT1 expression (p<0.01) compared to the control group. A marked decrease in Nestin expression was also observed in the 5 mg/kg (p<0.001) and 30 mg/kg (p<0.0001) treated groups. These findings demonstrate that transplacental administration of ruxolitinib modulates key markers involved in neuronal differentiation and gliogenesis in the developing mouse brain, suggesting its potential use in correcting imbalances in early brain development. |
| first_indexed | 2025-11-15T14:38:50Z |
| format | Article |
| id | upm-118733 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:38:50Z |
| publishDate | 2025 |
| publisher | Neurotak Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1187332025-07-23T02:08:13Z http://psasir.upm.edu.my/id/eprint/118733/ Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development Abdullah, Amirah Nabilah Fasihah Zamri, Auni Alias, Khairunnisa Azhar, Nur Munawwarah Ling, King Hwa Cheah, Pike See Zainal Abidin, Shahidee Ruxolitinib is a Janus kinase (JAK) inhibitor that inhibits the JAK/STAT signalling pathway by targeting JAK1 and JAK2, which are crucial for regulating astrogliogenesis. This study assessed the effect of ruxolitinib (5 and 30 mg/kg/day) on developing mouse brains by administering it to pregnant mice from E7.5 to E20.5. No adverse effects were observed in the treated mice. The brains of P1.5 pups were collected, and RNA was extracted to assess markers of neurogenesis and astrogliogenesis through RT-qPCR. The results revealed a significant decrease in Gfap expression (p<0.0001) in both ruxolitinib-treated groups compared to the control, indicating a suppression of astrogliogenesis. Additionally, S100β expression (p<0.05) was significantly reduced in the 30 mg/kg ruxolitinib-treated group. In contrast, the expression of neuronal markers vGLuT1 (p<0.01) and vGLuT2 (p<0.01) increased significantly in the 30 mg/kg treated group, suggesting enhanced neuronal differentiation. Furthermore, 5 and 30 mg/kg ruxolitinib-treated groups showed a significant increase in GAT1 expression (p<0.01) compared to the control group. A marked decrease in Nestin expression was also observed in the 5 mg/kg (p<0.001) and 30 mg/kg (p<0.0001) treated groups. These findings demonstrate that transplacental administration of ruxolitinib modulates key markers involved in neuronal differentiation and gliogenesis in the developing mouse brain, suggesting its potential use in correcting imbalances in early brain development. Neurotak Publishing 2025 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118733/1/118733.pdf Abdullah, Amirah Nabilah and Fasihah Zamri, Auni and Alias, Khairunnisa and Azhar, Nur Munawwarah and Ling, King Hwa and Cheah, Pike See and Zainal Abidin, Shahidee (2025) Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development. Neuroscience Research Notes, 8 (1). art. no. 352. pp. 1-7. ISSN 2576-828X https://neuroscirn.org/ojs/index.php/nrnotes/article/view/352 10.31117/neuroscirn.v8i1.352 |
| spellingShingle | Abdullah, Amirah Nabilah Fasihah Zamri, Auni Alias, Khairunnisa Azhar, Nur Munawwarah Ling, King Hwa Cheah, Pike See Zainal Abidin, Shahidee Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development |
| title | Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development |
| title_full | Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development |
| title_fullStr | Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development |
| title_full_unstemmed | Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development |
| title_short | Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development |
| title_sort | transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development |
| url | http://psasir.upm.edu.my/id/eprint/118733/ http://psasir.upm.edu.my/id/eprint/118733/ http://psasir.upm.edu.my/id/eprint/118733/ http://psasir.upm.edu.my/id/eprint/118733/1/118733.pdf |