1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model

Zebrafish (Danio rerio) is ideal for studying the effects of toxins like lead or plumbum (Pb) which persist in the environment and harm body systems when absorbed. Increasing Pb concentration could result in a higher mortality rate and alteration of behavior and metabolism. The present study evaluat...

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Main Authors: Chong, Siok Geok, Ismail, Intan Safinar, Chong, Chou Min, Mad Nasir, Nadiah, Saleh Hodin, Nur Atikah
Format: Article
Published: Taylor & Francis 2024
Online Access:http://psasir.upm.edu.my/id/eprint/118255/
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author Chong, Siok Geok
Ismail, Intan Safinar
Chong, Chou Min
Mad Nasir, Nadiah
Saleh Hodin, Nur Atikah
author_facet Chong, Siok Geok
Ismail, Intan Safinar
Chong, Chou Min
Mad Nasir, Nadiah
Saleh Hodin, Nur Atikah
author_sort Chong, Siok Geok
building UPM Institutional Repository
collection Online Access
description Zebrafish (Danio rerio) is ideal for studying the effects of toxins like lead or plumbum (Pb) which persist in the environment and harm body systems when absorbed. Increasing Pb concentration could result in a higher mortality rate and alteration of behavior and metabolism. The present study evaluates the acute toxicity effect of Pb on metabolome and behavior in adult zebrafish. The zebrafish were exposed to various Pb concentrations ranging from 0 to 30 mg/L for different periods (24, 48, and 72 h) before the fish samples were subjected to Nuclear Magnetic Resonance (NMR)-multivariate data analysis (MVDA) with additional support from behavioral assessment. The behavior of zebrafish was significantly altered after Pb inducement and the differential metabolites increased in low (5 mg/L) while decreased in high (10 mg/L) Pb concentrations. An ideal Pb induction could be achieved by 5 mg/L concentration in 24 h, which induced significant metabolite changes without irreversible damage. Continuing research on the effects of lead toxicity is crucial to develop effective prevention and treatment strategies.
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institution Universiti Putra Malaysia
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spelling upm-1182552025-07-02T03:37:41Z http://psasir.upm.edu.my/id/eprint/118255/ 1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model Chong, Siok Geok Ismail, Intan Safinar Chong, Chou Min Mad Nasir, Nadiah Saleh Hodin, Nur Atikah Zebrafish (Danio rerio) is ideal for studying the effects of toxins like lead or plumbum (Pb) which persist in the environment and harm body systems when absorbed. Increasing Pb concentration could result in a higher mortality rate and alteration of behavior and metabolism. The present study evaluates the acute toxicity effect of Pb on metabolome and behavior in adult zebrafish. The zebrafish were exposed to various Pb concentrations ranging from 0 to 30 mg/L for different periods (24, 48, and 72 h) before the fish samples were subjected to Nuclear Magnetic Resonance (NMR)-multivariate data analysis (MVDA) with additional support from behavioral assessment. The behavior of zebrafish was significantly altered after Pb inducement and the differential metabolites increased in low (5 mg/L) while decreased in high (10 mg/L) Pb concentrations. An ideal Pb induction could be achieved by 5 mg/L concentration in 24 h, which induced significant metabolite changes without irreversible damage. Continuing research on the effects of lead toxicity is crucial to develop effective prevention and treatment strategies. Taylor & Francis 2024-05-10 Article PeerReviewed Chong, Siok Geok and Ismail, Intan Safinar and Chong, Chou Min and Mad Nasir, Nadiah and Saleh Hodin, Nur Atikah (2024) 1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model. Drug and Chemical Toxicology, 47 (5). pp. 573-586. ISSN 0148-0545; eISSN: 1525-6014 https://www.tandfonline.com/doi/full/10.1080/01480545.2024.2346751 10.1080/01480545.2024.2346751
spellingShingle Chong, Siok Geok
Ismail, Intan Safinar
Chong, Chou Min
Mad Nasir, Nadiah
Saleh Hodin, Nur Atikah
1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model
title 1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model
title_full 1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model
title_fullStr 1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model
title_full_unstemmed 1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model
title_short 1H NMR-metabolomics studies on acute toxicity effect of lead in adult zebrafish (Danio rerio) model
title_sort 1h nmr-metabolomics studies on acute toxicity effect of lead in adult zebrafish (danio rerio) model
url http://psasir.upm.edu.my/id/eprint/118255/
http://psasir.upm.edu.my/id/eprint/118255/
http://psasir.upm.edu.my/id/eprint/118255/