Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies
This study aimed to analyse the effect of cocultivation between Lactococcus lactis ATCC 11454 with Lactobacillus rhamnosus ATCC 7469 through their growth profile, riboflavin and nisin production in 2.0 L stirred tank bioreactors. Batch fermentation was employed in the control (C) bioreactor, while f...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher Enterprise
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/117907/ http://psasir.upm.edu.my/id/eprint/117907/1/117907.pdf |
| _version_ | 1848867376606478336 |
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| author | Abdul Halid, Nadra Ibrahim, Mohamad Faizal Haryani, Yuli Mohd Zaini, Nurul Solehah Hasan, Hanan |
| author_facet | Abdul Halid, Nadra Ibrahim, Mohamad Faizal Haryani, Yuli Mohd Zaini, Nurul Solehah Hasan, Hanan |
| author_sort | Abdul Halid, Nadra |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study aimed to analyse the effect of cocultivation between Lactococcus lactis ATCC 11454 with Lactobacillus rhamnosus ATCC 7469 through their growth profile, riboflavin and nisin production in 2.0 L stirred tank bioreactors. Batch fermentation was employed in the control (C) bioreactor, while fed-batch fermentation employed in bioreactor run (B1) and run 2 (B2), with different medium flow rates of 10 mL/min and 1.6 mL/min, respectively. Results showed that B1 was able to generate an increase of 33.5 % in riboflavin production and a 36% growth rate compared to C and B2. The application in the fermentation process resulted in a prolonged exponential phase, subsequently contributing to a longer metabolite production timeframe. The findings from this study provided an efficient prospect of LAB co-culture, potentially by extending riboflavin production time and increasing vitamin levels in fermented foods or facilitating the production of valuable metabolites. |
| first_indexed | 2025-11-15T14:35:31Z |
| format | Article |
| id | upm-117907 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:35:31Z |
| publishDate | 2024 |
| publisher | Hibiscus Publisher Enterprise |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1179072025-06-16T08:03:25Z http://psasir.upm.edu.my/id/eprint/117907/ Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies Abdul Halid, Nadra Ibrahim, Mohamad Faizal Haryani, Yuli Mohd Zaini, Nurul Solehah Hasan, Hanan This study aimed to analyse the effect of cocultivation between Lactococcus lactis ATCC 11454 with Lactobacillus rhamnosus ATCC 7469 through their growth profile, riboflavin and nisin production in 2.0 L stirred tank bioreactors. Batch fermentation was employed in the control (C) bioreactor, while fed-batch fermentation employed in bioreactor run (B1) and run 2 (B2), with different medium flow rates of 10 mL/min and 1.6 mL/min, respectively. Results showed that B1 was able to generate an increase of 33.5 % in riboflavin production and a 36% growth rate compared to C and B2. The application in the fermentation process resulted in a prolonged exponential phase, subsequently contributing to a longer metabolite production timeframe. The findings from this study provided an efficient prospect of LAB co-culture, potentially by extending riboflavin production time and increasing vitamin levels in fermented foods or facilitating the production of valuable metabolites. Hibiscus Publisher Enterprise 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/117907/1/117907.pdf Abdul Halid, Nadra and Ibrahim, Mohamad Faizal and Haryani, Yuli and Mohd Zaini, Nurul Solehah and Hasan, Hanan (2024) Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies. Journal of Biochemistry, Microbiology and Biotechnology, 12 (spec.1). pp. 96-99. ISSN 2289-5779 https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/954 10.54987/jobimb.v12isp1.954 |
| spellingShingle | Abdul Halid, Nadra Ibrahim, Mohamad Faizal Haryani, Yuli Mohd Zaini, Nurul Solehah Hasan, Hanan Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies |
| title | Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies |
| title_full | Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies |
| title_fullStr | Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies |
| title_full_unstemmed | Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies |
| title_short | Scaling up co-cultivation fermentation of Lactococcus lactis ATCC 11454 and Lactobacillus rhamnosus ATCC 7469 with various feeding strategies |
| title_sort | scaling up co-cultivation fermentation of lactococcus lactis atcc 11454 and lactobacillus rhamnosus atcc 7469 with various feeding strategies |
| url | http://psasir.upm.edu.my/id/eprint/117907/ http://psasir.upm.edu.my/id/eprint/117907/ http://psasir.upm.edu.my/id/eprint/117907/ http://psasir.upm.edu.my/id/eprint/117907/1/117907.pdf |