In vitro maturation and fertilization of swamp buffalo (Bubalus bubalis) oocytes
Cumulus-oocyte complexes (COC) collected at slaughter from adult (n=4) and gonadotrophin-treated prepuberal (n=4) swamp buffaloes were matured in TCM199 with fetal calf serum (FCS), antibiotics and hormones (follicle stimulating hormone (FSH) and E2), at 39°C in 5% CO2 and 95% air for 24 h. Freshly...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier Science B.V.
1993
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116756/ http://psasir.upm.edu.my/id/eprint/116756/1/116756.pdf |