Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction
Lipase from Candida rugosa was immobilized by entrapment on poly(N- vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) (poly[VP-co-HEMA]) hydrogel, and divinylbenzene was the crosslinking agent. The immobilized enzymes were used in the esterification reaction of oleic acid and butanol in hexane. Th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Humana Press
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116526/ http://psasir.upm.edu.my/id/eprint/116526/1/116526.pdf |
| _version_ | 1848867025662771200 |
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| author | Basri, Mahiran Samsudin, Sunana Ahmad, Mansor Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_facet | Basri, Mahiran Samsudin, Sunana Ahmad, Mansor Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_sort | Basri, Mahiran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lipase from Candida rugosa was immobilized by entrapment on poly(N- vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) (poly[VP-co-HEMA]) hydrogel, and divinylbenzene was the crosslinking agent. The immobilized enzymes were used in the esterification reaction of oleic acid and butanol in hexane. The activities of the immobilized enzymes and the leaching ability of the enzyme from the support with respect to the different compositions of the hydrogels were investigated. The thermal, solvent, and storage stability of the immobilized lipases was also determined. Increasing the percentage of composition of VP from 0 to 90, which corresponds to the increase in the hydrophilicity of the hydrogels, increased the activity of the immobilized enzyme. Lipase immobilized on VP(%):HEMA(%) 90:10 exhibited the highest activity. Lipase immobilized on VP(%):HEMA(%) 50:50 showed the highest thermal, solvent, storage, and operational stability compared to lipase immobilized on other compositions of hydrogels as well as the native lipase. |
| first_indexed | 2025-11-15T14:29:56Z |
| format | Article |
| id | upm-116526 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:29:56Z |
| publishDate | 1999 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1165262025-04-10T04:22:04Z http://psasir.upm.edu.my/id/eprint/116526/ Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction Basri, Mahiran Samsudin, Sunana Ahmad, Mansor Abdul Razak, Che Nyonya Salleh, Abu Bakar Lipase from Candida rugosa was immobilized by entrapment on poly(N- vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) (poly[VP-co-HEMA]) hydrogel, and divinylbenzene was the crosslinking agent. The immobilized enzymes were used in the esterification reaction of oleic acid and butanol in hexane. The activities of the immobilized enzymes and the leaching ability of the enzyme from the support with respect to the different compositions of the hydrogels were investigated. The thermal, solvent, and storage stability of the immobilized lipases was also determined. Increasing the percentage of composition of VP from 0 to 90, which corresponds to the increase in the hydrophilicity of the hydrogels, increased the activity of the immobilized enzyme. Lipase immobilized on VP(%):HEMA(%) 90:10 exhibited the highest activity. Lipase immobilized on VP(%):HEMA(%) 50:50 showed the highest thermal, solvent, storage, and operational stability compared to lipase immobilized on other compositions of hydrogels as well as the native lipase. Humana Press 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116526/1/116526.pdf Basri, Mahiran and Samsudin, Sunana and Ahmad, Mansor and Abdul Razak, Che Nyonya and Salleh, Abu Bakar (1999) Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 81 (3). pp. 205-217. ISSN 0273-2289; eISSN: 0273-2289 https://link.springer.com/article/10.1385/ABAB:81:3:205?error=cookies_not_supported&code=40e7f548-b15e-4242-8246-3afb419d2de3 10.1385/abab:81:3:205 |
| spellingShingle | Basri, Mahiran Samsudin, Sunana Ahmad, Mansor Abdul Razak, Che Nyonya Salleh, Abu Bakar Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction |
| title | Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction |
| title_full | Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction |
| title_fullStr | Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction |
| title_full_unstemmed | Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction |
| title_short | Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction |
| title_sort | lipase immobilized on poly(vp-co-hema) hydrogel for esterification reaction |
| url | http://psasir.upm.edu.my/id/eprint/116526/ http://psasir.upm.edu.my/id/eprint/116526/ http://psasir.upm.edu.my/id/eprint/116526/ http://psasir.upm.edu.my/id/eprint/116526/1/116526.pdf |