Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution
Microalgae are promising natural antioxidant resources because they adapt to oxidative stresses in harsh oceanic conditions. Limited studies have explored the correlation between antioxidant activities and nutrients or exogenous factors that elevate superoxide dismutase (SOD) levels. Tetraselmis chu...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116318/ http://psasir.upm.edu.my/id/eprint/116318/1/116318.pdf |
| _version_ | 1848866975445417984 |
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| author | Khayat, Mohd Ezuan Halim, Murni Huang, Zhen Ni |
| author_facet | Khayat, Mohd Ezuan Halim, Murni Huang, Zhen Ni |
| author_sort | Khayat, Mohd Ezuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Microalgae are promising natural antioxidant resources because they adapt to oxidative stresses in harsh oceanic conditions. Limited studies have explored the correlation between antioxidant activities and nutrients or exogenous factors that elevate superoxide dismutase (SOD) levels. Tetraselmis chuii, a marine microalga with high SOD bioactivity, was recently listed as a novel food by the EU Commission. This study aimed to, at first, optimize the production medium to enhance biomass and SOD production in T. chuii, followed by stress induction to further boost SOD bioproduction. The Adaptive Laboratory Evolution (ALE) technique was employed to adapt T. chuii under stressed conditions for SOD enrichment. A two-stage cultivation process was designed: the first stage involved statistical optimization of the cultivation medium to increase biomass. In contrast, the second stage applied various stresses at the late exponential growth phase to induce oxidative stress and trigger SOD production as a defense mechanism. |
| first_indexed | 2025-11-15T14:29:08Z |
| format | Conference or Workshop Item |
| id | upm-116318 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:29:08Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1163182025-03-25T02:07:03Z http://psasir.upm.edu.my/id/eprint/116318/ Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution Khayat, Mohd Ezuan Halim, Murni Huang, Zhen Ni Microalgae are promising natural antioxidant resources because they adapt to oxidative stresses in harsh oceanic conditions. Limited studies have explored the correlation between antioxidant activities and nutrients or exogenous factors that elevate superoxide dismutase (SOD) levels. Tetraselmis chuii, a marine microalga with high SOD bioactivity, was recently listed as a novel food by the EU Commission. This study aimed to, at first, optimize the production medium to enhance biomass and SOD production in T. chuii, followed by stress induction to further boost SOD bioproduction. The Adaptive Laboratory Evolution (ALE) technique was employed to adapt T. chuii under stressed conditions for SOD enrichment. A two-stage cultivation process was designed: the first stage involved statistical optimization of the cultivation medium to increase biomass. In contrast, the second stage applied various stresses at the late exponential growth phase to induce oxidative stress and trigger SOD production as a defense mechanism. 2024 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116318/1/116318.pdf Khayat, Mohd Ezuan and Halim, Murni and Huang, Zhen Ni (2024) Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution. In: Biotech Horizon 2024 International Conference, 24-26 Sep. 2024, Universiti Putra Malaysia, Serdang. (p. 61). |
| spellingShingle | Khayat, Mohd Ezuan Halim, Murni Huang, Zhen Ni Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution |
| title | Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution |
| title_full | Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution |
| title_fullStr | Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution |
| title_full_unstemmed | Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution |
| title_short | Optimizing biomass and superoxide dismutase in Tetraselmis chuii through stress induction and adaptive laboratory evolution |
| title_sort | optimizing biomass and superoxide dismutase in tetraselmis chuii through stress induction and adaptive laboratory evolution |
| url | http://psasir.upm.edu.my/id/eprint/116318/ http://psasir.upm.edu.my/id/eprint/116318/1/116318.pdf |