Novel recombinant cold-adapted alkaliphilic lipase (Glalip03) from Antarctic yeast, Glaciozyma antarctica PI12
The present study investigates a novel low-temperature adapted lipase, Glalip03, from Glaciozyma antarctica. The gene was codon-optimised, synthesised, and heterologously expressed in E. coli strain Origami™ B (DE3) as a fusion with thioredoxin (Trx) using a pET32b (+) expression vector. The recombi...
| Main Authors: | Matinja, Adamu Idris, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Oslan, Siti Nurbaya |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media Deutschland
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/115733/ http://psasir.upm.edu.my/id/eprint/115733/1/115733.pdf |
Similar Items
Structural insights into cold-active lipase from Glaciozyma antarctica PI12: AlphaFold2 prediction and molecular dynamics simulation
by: Matinja, Adamu Idris, et al.
Published: (2024)
by: Matinja, Adamu Idris, et al.
Published: (2024)
Molecular cloning and optimization for high level expression of cold-adapted serine protease from antarctic yeast glaciozyma antarctica PI12
by: Alias, Norsyuhada, et al.
Published: (2014)
by: Alias, Norsyuhada, et al.
Published: (2014)
Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris
by: Md Tab M.,, et al.
Published: (2017)
by: Md Tab M.,, et al.
Published: (2017)
Bioinformatics survey of the metal usage by psychrophilic yeast Glaciozyma antarctica PI12
by: Foong, Pik Mun, et al.
Published: (2015)
by: Foong, Pik Mun, et al.
Published: (2015)
Genome mining for glycoside hydrolases from the
psychrophilic yeast Glaciozyma antarctica PI12
by: Nooraisyah, M.N., et al.
Published: (2015)
by: Nooraisyah, M.N., et al.
Published: (2015)
(-)-glaciantarcin, a new dipeptide and some secondary metabolites
from the psychrophilic yeast Glaciozyma antarctica PI12
by: Andi R. Rosandy,, et al.
Published: (2018)
by: Andi R. Rosandy,, et al.
Published: (2018)
In silico analysis and 3D structure prediction
of a chitinase from psychrophilic yeast
Glaciozyma antarctica PI12
by: Yusof N.Y.,, et al.
Published: (2017)
by: Yusof N.Y.,, et al.
Published: (2017)
Cold-active lipases and esterases: a review on recombinant overexpression and other essential issues
by: Matinja, Adamu Idris, et al.
Published: (2022)
by: Matinja, Adamu Idris, et al.
Published: (2022)
Exploring the protease diversity of psychrophilic yeast, glaciozyma antarctica through genome mining analysis
by: Norfarhan Mohd-Assaad,, et al.
Published: (2024)
by: Norfarhan Mohd-Assaad,, et al.
Published: (2024)
Comparison of rna extraction methods for
transcript analysis from the psychrophilic yeast,
Glaciozyma antarctica
by: Izwan, B., et al.
Published: (2014)
by: Izwan, B., et al.
Published: (2014)
Immobilization and characterization of hormone-sensitive lipase (HSL) from Glaciozyma antarctica
by: Ishak, Siti Nor Hasmah, et al.
Published: (2024)
by: Ishak, Siti Nor Hasmah, et al.
Published: (2024)
Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12
by: Alias, Norsyuhada, et al.
Published: (2014)
by: Alias, Norsyuhada, et al.
Published: (2014)
Use of a newly isolated yeast in the expression of recombinant proteins
by: Salleh, Abu Bakar, et al.
Published: (2012)
by: Salleh, Abu Bakar, et al.
Published: (2012)
A newly isolated yeast as an expression host for recombinant lipase
by: Oslan, Siti Nurbaya, et al.
Published: (2015)
by: Oslan, Siti Nurbaya, et al.
Published: (2015)
Structural and Functional Analysis of a Novel Psychrophilic
β-mannanase from Glaciozyma antarctica PI12
by: Parvizpour, Sepideh, et al.
Published: (2014)
by: Parvizpour, Sepideh, et al.
Published: (2014)
Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions
by: Nurul Sarah Izzati Zahid,, et al.
Published: (2025)
by: Nurul Sarah Izzati Zahid,, et al.
Published: (2025)
Expression, characterisation and homology modelling of a novel hormone-sensitive lipase (HSL)-like esterase from Glaciozyma antarctica
by: Mohamad Tahir, Hiryahafira, et al.
Published: (2020)
by: Mohamad Tahir, Hiryahafira, et al.
Published: (2020)
New indolizidine alkaloids from psychrophilic yeast Glyciozyma antarctica PI12
by: Rosandy, Andi Rifki, et al.
Published: (2016)
by: Rosandy, Andi Rifki, et al.
Published: (2016)
In silico characterisation of the Glaciozyma antarctica
Genome: mining the molecular chaperones
by: Nur Athirah, Y., et al.
Published: (2015)
by: Nur Athirah, Y., et al.
Published: (2015)
Structural prediction and cloning of small, haloacid dehalogenase-like protein of glaciozyma antarctica PI12 in pichia pastoris GS115
by: Pauzi, Syaidatul Syazana
Published: (2015)
by: Pauzi, Syaidatul Syazana
Published: (2015)
Expression, characterization and homology modelling of a novel hormone-sensitive lipase like-esterase from Glaciozyma antarctica PI12
by: Mohamad Tahir, Hiryahafira
Published: (2020)
by: Mohamad Tahir, Hiryahafira
Published: (2020)
Molecular expression of a recombinant thermostable bacterial amylase from geobacillus stearothermophilus SR74 using methanol-free meyerozyma guilliermondii strain SO yeast system
by: Mohamad Nasir, Nurul Syazwani, et al.
Published: (2020)
by: Mohamad Nasir, Nurul Syazwani, et al.
Published: (2020)
Critical physical parameters for optimum recombinant protein production in yeast systems
by: Pallujam, Asha Devi, et al.
Published: (2020)
by: Pallujam, Asha Devi, et al.
Published: (2020)
Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
by: Eskandari, Azadeh, et al.
Published: (2024)
by: Eskandari, Azadeh, et al.
Published: (2024)
Bioinformatic analyses of metallome and structural analysis of sirtuin from Glaciozyma antarctica PII2
by: Foong, Pik Mun
Published: (2014)
by: Foong, Pik Mun
Published: (2014)
A Cold Active Bifunctional Lipase with Protease Activity from Antarctic Microorganism Pi 12
by: Mohamad Ali, Mohd Shukuri
Published: (2009)
by: Mohamad Ali, Mohd Shukuri
Published: (2009)
Pengenalpastian dan profil pengekspresan gen biosintesis asid amino yis psikrofil, Glaciozyma antarctica
by: Izwan Bharudin,, et al.
Published: (2018)
by: Izwan Bharudin,, et al.
Published: (2018)
Structure and function of novel antifreeze peptides derived from Glaciozyma antarctica antifreeze protein-1
by: Said Amin Shah, Syed Hussinien Hielmie Shah
Published: (2013)
by: Said Amin Shah, Syed Hussinien Hielmie Shah
Published: (2013)
Development of yeast expression systems for producing thermostable T1 lipase
by: Oslan, Siti Nurbaya
Published: (2012)
by: Oslan, Siti Nurbaya
Published: (2012)
Cold-adapted organic solvent tolerant alkalophilic family I.3 lipase from an Antarctic Pseudomonas
by: Ganasen, Menega, et al.
Published: (2016)
by: Ganasen, Menega, et al.
Published: (2016)
Production of lipases in yeast expression system
by: Shahidan, Noor Hidayah, et al.
Published: (2013)
by: Shahidan, Noor Hidayah, et al.
Published: (2013)
Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
by: Eskandari, Azadeh, et al.
Published: (2024)
by: Eskandari, Azadeh, et al.
Published: (2024)
Reverse micellar extraction of a recombinant cold-adapted AMs8 lipase from the antarctic Pseudomonas fluorescens
by: Abd. Jalil, Fatin Nur Fauzi Ana
Published: (2019)
by: Abd. Jalil, Fatin Nur Fauzi Ana
Published: (2019)
Cold adaptation strategies and diversity of Antarctic bacteria
by: Gilbert, Jack Anthony
Published: (2002)
by: Gilbert, Jack Anthony
Published: (2002)
Cold-adapted RTX lipase from antarctic Pseudomonas sp.
strain AMS8: isolation, molecular modeling and heterologous
expression
by: Mohamad Ali, Mohd Shukuri, et al.
Published: (2013)
by: Mohamad Ali, Mohd Shukuri, et al.
Published: (2013)
Expression and characterization of Geobacillus stearothermophilus SR74 recombinant a-Amylase in Pichia pastoris
by: Gandhi, Sivasangkary, et al.
Published: (2015)
by: Gandhi, Sivasangkary, et al.
Published: (2015)
Unsaturated fatty acids in antarctic bacteria
by: Garba, Lawal, et al.
Published: (2016)
by: Garba, Lawal, et al.
Published: (2016)
Production and characterisation of a recombinant cold adapted lipase from Pseudomonas sp. LSK25 isolated from Signy Station, Antarctica
by: Salwoom, Leelatulasi
Published: (2019)
by: Salwoom, Leelatulasi
Published: (2019)
Biodegradation of phenol by cold-adapted bacteria isolated from antarctic soils
by: Lee, Gillian Li Yin
Published: (2018)
by: Lee, Gillian Li Yin
Published: (2018)
Enzymatic Deinking Using Thermostable And Alkaliphilic Endoglucanase Of Indigenous Bacteria
by: Dayang Syahreeny, Abang Mustafa
Published: (2010)
by: Dayang Syahreeny, Abang Mustafa
Published: (2010)
Similar Items
-
Structural insights into cold-active lipase from Glaciozyma antarctica PI12: AlphaFold2 prediction and molecular dynamics simulation
by: Matinja, Adamu Idris, et al.
Published: (2024) -
Molecular cloning and optimization for high level expression of cold-adapted serine protease from antarctic yeast glaciozyma antarctica PI12
by: Alias, Norsyuhada, et al.
Published: (2014) -
Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris
by: Md Tab M.,, et al.
Published: (2017) -
Bioinformatics survey of the metal usage by psychrophilic yeast Glaciozyma antarctica PI12
by: Foong, Pik Mun, et al.
Published: (2015) -
Genome mining for glycoside hydrolases from the
psychrophilic yeast Glaciozyma antarctica PI12
by: Nooraisyah, M.N., et al.
Published: (2015)