Stability of hydrophobic lipase derivatives immobilized on organic polymer beads
Lipase from Candida rugosa was immobilized by attaching various hydrophobic groups to the enzyme molecule and adsorbing these hydrophobic lipase derivatives on several organic polymer beads. The immobilized enzymes were more thermostable in organic solvents compared to the native and modified Upases...
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| Format: | Article |
| Language: | English |
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Humana Press
1994
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114810/ http://psasir.upm.edu.my/id/eprint/114810/1/114810.pdf |
| _version_ | 1848866602048552960 |
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| author | Basri, M. Ampon, K. Wan Yunus, W.M.Z. A. Razak, C.N. |
| author_facet | Basri, M. Ampon, K. Wan Yunus, W.M.Z. A. Razak, C.N. |
| author_sort | Basri, M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lipase from Candida rugosa was immobilized by attaching various hydrophobic groups to the enzyme molecule and adsorbing these hydrophobic lipase derivatives on several organic polymer beads. The immobilized enzymes were more thermostable in organic solvents compared to the native and modified Upases. Thermostability was highest with ΧAD2 beads, followed by ΧAD7 and RCOOH. Initially modifying the enzyme with hydrophobic modifiers did not have any effect on the enzyme thermostability. The best conditions for storing these enzyme preparations were at very low temperature in the lyophilized form and in a solution containing the reaction substrate. Interestingly, PEG-lipase immobilized on ΧAD7 beads showed increased operational stability when used in a stirred-tank reactor. The operational stability was further increased by a mild glutaraldehyde treatment of the enzyme preparation. © 1994 Humana Press Inc. |
| first_indexed | 2025-11-15T14:23:12Z |
| format | Article |
| id | upm-114810 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:23:12Z |
| publishDate | 1994 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1148102025-02-03T04:17:09Z http://psasir.upm.edu.my/id/eprint/114810/ Stability of hydrophobic lipase derivatives immobilized on organic polymer beads Basri, M. Ampon, K. Wan Yunus, W.M.Z. A. Razak, C.N. Lipase from Candida rugosa was immobilized by attaching various hydrophobic groups to the enzyme molecule and adsorbing these hydrophobic lipase derivatives on several organic polymer beads. The immobilized enzymes were more thermostable in organic solvents compared to the native and modified Upases. Thermostability was highest with ΧAD2 beads, followed by ΧAD7 and RCOOH. Initially modifying the enzyme with hydrophobic modifiers did not have any effect on the enzyme thermostability. The best conditions for storing these enzyme preparations were at very low temperature in the lyophilized form and in a solution containing the reaction substrate. Interestingly, PEG-lipase immobilized on ΧAD7 beads showed increased operational stability when used in a stirred-tank reactor. The operational stability was further increased by a mild glutaraldehyde treatment of the enzyme preparation. © 1994 Humana Press Inc. Humana Press 1994 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114810/1/114810.pdf Basri, M. and Ampon, K. and Wan Yunus, W.M.Z. and A. Razak, C.N. (1994) Stability of hydrophobic lipase derivatives immobilized on organic polymer beads. Applied Biochemistry and Biotechnology, 48 (3). pp. 173-183. ISSN 1559-0291; eISSN: 0273-2289 https://link.springer.com/article/10.1007/BF02788740?error=cookies_not_supported&code=0f51ffbe-4fd5-4b76-8185-9c1b123c2738 10.1007/BF02788740 |
| spellingShingle | Basri, M. Ampon, K. Wan Yunus, W.M.Z. A. Razak, C.N. Stability of hydrophobic lipase derivatives immobilized on organic polymer beads |
| title | Stability of hydrophobic lipase derivatives immobilized on organic polymer beads |
| title_full | Stability of hydrophobic lipase derivatives immobilized on organic polymer beads |
| title_fullStr | Stability of hydrophobic lipase derivatives immobilized on organic polymer beads |
| title_full_unstemmed | Stability of hydrophobic lipase derivatives immobilized on organic polymer beads |
| title_short | Stability of hydrophobic lipase derivatives immobilized on organic polymer beads |
| title_sort | stability of hydrophobic lipase derivatives immobilized on organic polymer beads |
| url | http://psasir.upm.edu.my/id/eprint/114810/ http://psasir.upm.edu.my/id/eprint/114810/ http://psasir.upm.edu.my/id/eprint/114810/ http://psasir.upm.edu.my/id/eprint/114810/1/114810.pdf |