Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines

New synthetic routes to a series of tetra- and pentacyclic acridines related in structure to marine natural products are reported. The novel water-soluble agent dihydroindolizino[7,6,5-kl]acridinium chloride 14 has inhibitory activity in a panel of non-small-cell lung and breast tumor cell lines exc...

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Main Authors: Stanslas, Johnson, Hagan, Damien J., Ellis, Michael J., Turner, Claire, Carmichael, James, Ward, Wynne, Hammonds, Timothy R., Stevens, Malcolm F. G.
Format: Article
Published: American Chemical Society 2000
Online Access:http://psasir.upm.edu.my/id/eprint/114416/
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author Stanslas, Johnson
Hagan, Damien J.
Ellis, Michael J.
Turner, Claire
Carmichael, James
Ward, Wynne
Hammonds, Timothy R.
Stevens, Malcolm F. G.
author_facet Stanslas, Johnson
Hagan, Damien J.
Ellis, Michael J.
Turner, Claire
Carmichael, James
Ward, Wynne
Hammonds, Timothy R.
Stevens, Malcolm F. G.
author_sort Stanslas, Johnson
building UPM Institutional Repository
collection Online Access
description New synthetic routes to a series of tetra- and pentacyclic acridines related in structure to marine natural products are reported. The novel water-soluble agent dihydroindolizino[7,6,5-kl]acridinium chloride 14 has inhibitory activity in a panel of non-small-cell lung and breast tumor cell lines exceeding that of m-AMSA. The salt inhibited the release of minicircle products of kDNA confirming that disorganization of topoisomerase II partly underlies the activity of the compound. COMPARE analysis of the NCI mean graph profile of compound 14 at the GI50 level corroborates this conclusion with Pearson correlation coefficients (> 0.6) to clinical agents of the topoisomerase II class: however, this correlation was not seen at the LC50 level. The inhibitory action of 14 on Saccharomyces cerevisiae transfected with human topoisomerase II isoforms showed a 3-fold selectivity against the IIα isoform over the IIβ isoform. Unlike m-AMSA, 14 is not susceptible to P-glycoprotein-mediated drug efflux and retains activity in lung cells with derived resistance to the topoisomerase II inhibitor etoposide.
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spelling upm-1144162025-01-15T02:32:16Z http://psasir.upm.edu.my/id/eprint/114416/ Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines Stanslas, Johnson Hagan, Damien J. Ellis, Michael J. Turner, Claire Carmichael, James Ward, Wynne Hammonds, Timothy R. Stevens, Malcolm F. G. New synthetic routes to a series of tetra- and pentacyclic acridines related in structure to marine natural products are reported. The novel water-soluble agent dihydroindolizino[7,6,5-kl]acridinium chloride 14 has inhibitory activity in a panel of non-small-cell lung and breast tumor cell lines exceeding that of m-AMSA. The salt inhibited the release of minicircle products of kDNA confirming that disorganization of topoisomerase II partly underlies the activity of the compound. COMPARE analysis of the NCI mean graph profile of compound 14 at the GI50 level corroborates this conclusion with Pearson correlation coefficients (> 0.6) to clinical agents of the topoisomerase II class: however, this correlation was not seen at the LC50 level. The inhibitory action of 14 on Saccharomyces cerevisiae transfected with human topoisomerase II isoforms showed a 3-fold selectivity against the IIα isoform over the IIβ isoform. Unlike m-AMSA, 14 is not susceptible to P-glycoprotein-mediated drug efflux and retains activity in lung cells with derived resistance to the topoisomerase II inhibitor etoposide. American Chemical Society 2000 Article PeerReviewed Stanslas, Johnson and Hagan, Damien J. and Ellis, Michael J. and Turner, Claire and Carmichael, James and Ward, Wynne and Hammonds, Timothy R. and Stevens, Malcolm F. G. (2000) Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines. Journal of Medicinal Chemistry, 43 (8). pp. 1563-1572. ISSN 0022-2623; eISSN: 0022-2623 https://pubs.acs.org/doi/10.1021/jm9909490 10.1021/jm9909490
spellingShingle Stanslas, Johnson
Hagan, Damien J.
Ellis, Michael J.
Turner, Claire
Carmichael, James
Ward, Wynne
Hammonds, Timothy R.
Stevens, Malcolm F. G.
Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines
title Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines
title_full Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines
title_fullStr Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines
title_full_unstemmed Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines
title_short Antitumor polycyclic acridines. 7. Synthesis and biological properties of DNA affinic tetra- and pentacyclic acridines
title_sort antitumor polycyclic acridines. 7. synthesis and biological properties of dna affinic tetra- and pentacyclic acridines
url http://psasir.upm.edu.my/id/eprint/114416/
http://psasir.upm.edu.my/id/eprint/114416/
http://psasir.upm.edu.my/id/eprint/114416/