Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep
We have previously demonstrated that pre- and early postnatal malnutrition in sheep induced depot- and sex-specific changes in adipose morphological features, metabolic outcomes, and transcriptome in adulthood, with perirenal (PER) as the major target followed by subcutaneous (SUB) adipose tissue. W...
| Main Authors: | , , , , |
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| Format: | Article |
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American Physiological Society
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108086/ |
| _version_ | 1848865069208698880 |
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| author | Ahmad, Sharmila Drag, Markus Hodal Mohamad Salleh, Suraya Cai, Zexi Nielsen, Mette Olaf |
| author_facet | Ahmad, Sharmila Drag, Markus Hodal Mohamad Salleh, Suraya Cai, Zexi Nielsen, Mette Olaf |
| author_sort | Ahmad, Sharmila |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | We have previously demonstrated that pre- and early postnatal malnutrition in sheep induced depot- and sex-specific changes in adipose morphological features, metabolic outcomes, and transcriptome in adulthood, with perirenal (PER) as the major target followed by subcutaneous (SUB) adipose tissue. We aimed to identify coexpressed and hub genes in SUB and PER to identify the underlying molecular mechanisms contributing to the early nutritional programming of adipose-related phenotypic outcomes. Transcriptomes of SUB and PER of male and female adult sheep with different pre- and early postnatal nutrition histories were used to construct networks of coexpressed genes likely to be functionally associated with pre- and early postnatal nutrition histories and phenotypic traits using weighted gene coexpression network analysis. The modules from PER showed enrichment of cell cycle regulation, gene expression, transmembrane transport, and metabolic processes associated with both sexes’ prenatal nutrition. In SUB (only males), a module of enriched adenosine diphosphate metabolism and development correlated with prenatal nutrition. Sex-specific module enrichments were found in PER, such as chromatin modification in the male network but histone modification and mitochondria- and oxidative phosphorylation-related functions in the female network. These sex-specific modules correlated with prenatal nutrition and adipocyte size distribution patterns. Our results point to PER as a primary target of prenatal malnutrition compared to SUB, which played only a minor role. The prenatal programming of gene expression and cell cycle, potentially through epigenetic modifications, might be underlying mechanisms responsible for observed changes in PER expandability and adipocyte-size distribution patterns in adulthood in both sexes. |
| first_indexed | 2025-11-15T13:58:50Z |
| format | Article |
| id | upm-108086 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:58:50Z |
| publishDate | 2023 |
| publisher | American Physiological Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1080862024-09-26T04:17:37Z http://psasir.upm.edu.my/id/eprint/108086/ Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep Ahmad, Sharmila Drag, Markus Hodal Mohamad Salleh, Suraya Cai, Zexi Nielsen, Mette Olaf We have previously demonstrated that pre- and early postnatal malnutrition in sheep induced depot- and sex-specific changes in adipose morphological features, metabolic outcomes, and transcriptome in adulthood, with perirenal (PER) as the major target followed by subcutaneous (SUB) adipose tissue. We aimed to identify coexpressed and hub genes in SUB and PER to identify the underlying molecular mechanisms contributing to the early nutritional programming of adipose-related phenotypic outcomes. Transcriptomes of SUB and PER of male and female adult sheep with different pre- and early postnatal nutrition histories were used to construct networks of coexpressed genes likely to be functionally associated with pre- and early postnatal nutrition histories and phenotypic traits using weighted gene coexpression network analysis. The modules from PER showed enrichment of cell cycle regulation, gene expression, transmembrane transport, and metabolic processes associated with both sexes’ prenatal nutrition. In SUB (only males), a module of enriched adenosine diphosphate metabolism and development correlated with prenatal nutrition. Sex-specific module enrichments were found in PER, such as chromatin modification in the male network but histone modification and mitochondria- and oxidative phosphorylation-related functions in the female network. These sex-specific modules correlated with prenatal nutrition and adipocyte size distribution patterns. Our results point to PER as a primary target of prenatal malnutrition compared to SUB, which played only a minor role. The prenatal programming of gene expression and cell cycle, potentially through epigenetic modifications, might be underlying mechanisms responsible for observed changes in PER expandability and adipocyte-size distribution patterns in adulthood in both sexes. American Physiological Society 2023 Article PeerReviewed Ahmad, Sharmila and Drag, Markus Hodal and Mohamad Salleh, Suraya and Cai, Zexi and Nielsen, Mette Olaf (2023) Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep. Comparative Physiological Genomics, 55 (9). pp. 392-413. ISSN 1094-8341; ESSN: 1531-2267 https://journals.physiology.org/doi/full/10.1152/physiolgenomics.00128.2022 10.1152/physiolgenomics.00128.2022 |
| spellingShingle | Ahmad, Sharmila Drag, Markus Hodal Mohamad Salleh, Suraya Cai, Zexi Nielsen, Mette Olaf Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep |
| title | Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep |
| title_full | Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep |
| title_fullStr | Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep |
| title_full_unstemmed | Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep |
| title_short | Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep |
| title_sort | gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep |
| url | http://psasir.upm.edu.my/id/eprint/108086/ http://psasir.upm.edu.my/id/eprint/108086/ http://psasir.upm.edu.my/id/eprint/108086/ |