Enhancement in t1 lipase purification recovery using the novel construct pgex4t1/his-t1
The Geobacillus zalihae strain T1 produces a thermostable T1 lipase that could be used for industrial purposes. Previously, the GST-T1 lipase was purified through two chromatographic steps: affinity and ion exchange (IEX) but the recovery yield was only 33%. To improve the recovery yield to over 80%...
| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
Informa Uk Limited
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/107690/ |