A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds
Fruit seed is one of the by-products of fruit processing that is currently underexploited. In this study, the crude water extracts of guava, longan, and rockmelon seeds were hydrolyzed using enzymes bromelain and alcalase. Their yields (2.47–57.02), TPC (15.53–127.99 mg GAE/g), TFC (18.03–115.15 mg...
| Main Authors: | , , , , |
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| Format: | Article |
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Springer
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/106259/ |
| _version_ | 1848864729497337856 |
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| author | Hew, Peir Shinn Wong, Zhian Joe Wong, Min Feung Ng, Wen Jie Ee, Kah Yaw |
| author_facet | Hew, Peir Shinn Wong, Zhian Joe Wong, Min Feung Ng, Wen Jie Ee, Kah Yaw |
| author_sort | Hew, Peir Shinn |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Fruit seed is one of the by-products of fruit processing that is currently underexploited. In this study, the crude water extracts of guava, longan, and rockmelon seeds were hydrolyzed using enzymes bromelain and alcalase. Their yields (2.47–57.02), TPC (15.53–127.99 mg GAE/g), TFC (18.03–115.15 mg QE/g), protein (14.04–178.60 mg/g), and peptide (23.55–203.97 mg/g) contents were reported. Among the guava seed samples, bromelain hydrolysate (G2) had the highest FRAP (121.489 mg Fe(II)/g), ABTS (IC50 1.713 mg/mL) and DPPH (IC50 0.158 mg/mL) values. Similarly, bromelain hydrolysate of rockmelon seed (R2) exhibited higher FRAP (41.511 mg Fe(II)/g) and ABTS (IC50 2.605 mg/mL) values than the crude extract. However, longan seed crude extract (L1) exhibited higher antioxidant activity than its hydrolysates. Furthermore, chemometric analysis revealed multifaceted relationship among physicochemical and antioxidant parameters, in which L1, bromelain (L2) and alcalase hydrolysates (L3) of longan seed, guava seed crude extract (G1), and G2 associated positively with TPC, TFC, and reducing antioxidant power. Overall, longan and guava seed extracts (L1, G1) and hydrolysates (L2, L3, G2) can be potentially used as antioxidant ingredients for food product development. |
| first_indexed | 2025-11-15T13:53:26Z |
| format | Article |
| id | upm-106259 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:53:26Z |
| publishDate | 2024 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1062592024-05-12T10:49:04Z http://psasir.upm.edu.my/id/eprint/106259/ A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds Hew, Peir Shinn Wong, Zhian Joe Wong, Min Feung Ng, Wen Jie Ee, Kah Yaw Fruit seed is one of the by-products of fruit processing that is currently underexploited. In this study, the crude water extracts of guava, longan, and rockmelon seeds were hydrolyzed using enzymes bromelain and alcalase. Their yields (2.47–57.02), TPC (15.53–127.99 mg GAE/g), TFC (18.03–115.15 mg QE/g), protein (14.04–178.60 mg/g), and peptide (23.55–203.97 mg/g) contents were reported. Among the guava seed samples, bromelain hydrolysate (G2) had the highest FRAP (121.489 mg Fe(II)/g), ABTS (IC50 1.713 mg/mL) and DPPH (IC50 0.158 mg/mL) values. Similarly, bromelain hydrolysate of rockmelon seed (R2) exhibited higher FRAP (41.511 mg Fe(II)/g) and ABTS (IC50 2.605 mg/mL) values than the crude extract. However, longan seed crude extract (L1) exhibited higher antioxidant activity than its hydrolysates. Furthermore, chemometric analysis revealed multifaceted relationship among physicochemical and antioxidant parameters, in which L1, bromelain (L2) and alcalase hydrolysates (L3) of longan seed, guava seed crude extract (G1), and G2 associated positively with TPC, TFC, and reducing antioxidant power. Overall, longan and guava seed extracts (L1, G1) and hydrolysates (L2, L3, G2) can be potentially used as antioxidant ingredients for food product development. Springer 2024 Article PeerReviewed Hew, Peir Shinn and Wong, Zhian Joe and Wong, Min Feung and Ng, Wen Jie and Ee, Kah Yaw (2024) A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds. Journal of Food Measurement and Characterization. pp. 1-11. ISSN 2193-4126; ESSN: 2193-4134 (In Press) https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184500982&url=10.1007%2fs11694-024-02362-8&partnerID=40&md5=024366ff9784c0c0c4b3c2045f917c5e 10.1007/s11694-024-02362-8 |
| spellingShingle | Hew, Peir Shinn Wong, Zhian Joe Wong, Min Feung Ng, Wen Jie Ee, Kah Yaw A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds |
| title | A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds |
| title_full | A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds |
| title_fullStr | A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds |
| title_full_unstemmed | A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds |
| title_short | A comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds |
| title_sort | comparative study of physicochemical and antioxidant properties integrated with chemometrics on enzymatic hydrolysates of selected fruit seeds |
| url | http://psasir.upm.edu.my/id/eprint/106259/ http://psasir.upm.edu.my/id/eprint/106259/ http://psasir.upm.edu.my/id/eprint/106259/ |