Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production
Biohydrogen production via an anaerobic dark fermentation process at thermophilic conditions is recognized as an excellent biological method and more cost-effective due to its ability to perform without light energy and oxygen source. At thermophilic, this research aims to investigate the effect...
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/104092/ http://psasir.upm.edu.my/id/eprint/104092/1/NUR%20FARAHANA%20BT%20DZUL%20RASHIDI%20-%20IR.pdf |
| _version_ | 1848864191654395904 |
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| author | Dzul Rashidi, Nur Farahana |
| author_facet | Dzul Rashidi, Nur Farahana |
| author_sort | Dzul Rashidi, Nur Farahana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Biohydrogen production via an anaerobic dark fermentation process at thermophilic
conditions is recognized as an excellent biological method and more cost-effective due
to its ability to perform without light energy and oxygen source. At thermophilic, this
research aims to investigate the effect of bacterial immobilization on a matrix made of
alginate and chitosan co-polymerization towards biohydrogen production. In the first
objective, the effect of hydrogen production using granular activated carbon (GAC) as a
microbial support carrier in forming GAC-attached biofilm was evaluated based on
different amounts of sugar concentration as microbial feedstock. The comparison
between initial sugars loading was conducted in a water bath shaker at 120rpm in 800
ml working volume. The acclimatization was operated in a sequencing batch system at
a thermophilic temperature of 60oC and the initial feedstock was set at pH 6. The
fermentation process was continuously carried out until a steady state of biogas was
obtained and it showed the attached-biofilm system successfully stabilizing hydrogen
production after 40 days. The second objective involved the entrapment process in the
formation of GAC-attached biofilm using alginate and chitosan as carrier polymers in
the form of beads. Bacterial immobilization was done by entrapment of GAC-attached
biofilm into 0.5g,1g,2g,3g and 4g of alginate and chitosan respectively (GAC-Alg and
GAC-AlgC). The immobilized beads for both alginate and chitosan were conducted in
batch fermentation using a synthetic medium at a temperature of 60°C, pH 6.0 and in
200 ml working volume. The entrapment of GAC-attached biofilm provides good
support for microorganisms to grow and colonize where high bacterial loads were
observed under a scanning electron microscope (SEM). Lastly, in the final objective, this
research was conducted to assess the performance of GAC-Alg and GAC-AlgC
immobilized beads by using POME as a fermentation medium. It has been observed that
the GAC-Alg immobilized beads resulted in stable hydrogen production after 52 hours
with a consistent HPR of 1.02 mmol H₂/l.h and 1.83 mmol H2/l.h for GAC- AlgC.
Overall, this study showed the immobilization of bacteria-entrapped beads promising
approach to protect the bacteria colonization during the fermentation, thus retaining and promoting microbial growth and protecting the microbial from an unfavourable
environment. |
| first_indexed | 2025-11-15T13:44:54Z |
| format | Thesis |
| id | upm-104092 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:44:54Z |
| publishDate | 2022 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1040922023-07-07T03:03:19Z http://psasir.upm.edu.my/id/eprint/104092/ Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production Dzul Rashidi, Nur Farahana Biohydrogen production via an anaerobic dark fermentation process at thermophilic conditions is recognized as an excellent biological method and more cost-effective due to its ability to perform without light energy and oxygen source. At thermophilic, this research aims to investigate the effect of bacterial immobilization on a matrix made of alginate and chitosan co-polymerization towards biohydrogen production. In the first objective, the effect of hydrogen production using granular activated carbon (GAC) as a microbial support carrier in forming GAC-attached biofilm was evaluated based on different amounts of sugar concentration as microbial feedstock. The comparison between initial sugars loading was conducted in a water bath shaker at 120rpm in 800 ml working volume. The acclimatization was operated in a sequencing batch system at a thermophilic temperature of 60oC and the initial feedstock was set at pH 6. The fermentation process was continuously carried out until a steady state of biogas was obtained and it showed the attached-biofilm system successfully stabilizing hydrogen production after 40 days. The second objective involved the entrapment process in the formation of GAC-attached biofilm using alginate and chitosan as carrier polymers in the form of beads. Bacterial immobilization was done by entrapment of GAC-attached biofilm into 0.5g,1g,2g,3g and 4g of alginate and chitosan respectively (GAC-Alg and GAC-AlgC). The immobilized beads for both alginate and chitosan were conducted in batch fermentation using a synthetic medium at a temperature of 60°C, pH 6.0 and in 200 ml working volume. The entrapment of GAC-attached biofilm provides good support for microorganisms to grow and colonize where high bacterial loads were observed under a scanning electron microscope (SEM). Lastly, in the final objective, this research was conducted to assess the performance of GAC-Alg and GAC-AlgC immobilized beads by using POME as a fermentation medium. It has been observed that the GAC-Alg immobilized beads resulted in stable hydrogen production after 52 hours with a consistent HPR of 1.02 mmol H₂/l.h and 1.83 mmol H2/l.h for GAC- AlgC. Overall, this study showed the immobilization of bacteria-entrapped beads promising approach to protect the bacteria colonization during the fermentation, thus retaining and promoting microbial growth and protecting the microbial from an unfavourable environment. 2022-06 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/104092/1/NUR%20FARAHANA%20BT%20DZUL%20RASHIDI%20-%20IR.pdf Dzul Rashidi, Nur Farahana (2022) Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production. Masters thesis, Universiti Putra Malaysia. Biofilms Carbon, Activated Microbial polysaccharides |
| spellingShingle | Biofilms Carbon, Activated Microbial polysaccharides Dzul Rashidi, Nur Farahana Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title | Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_full | Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_fullStr | Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_full_unstemmed | Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_short | Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_sort | evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| topic | Biofilms Carbon, Activated Microbial polysaccharides |
| url | http://psasir.upm.edu.my/id/eprint/104092/ http://psasir.upm.edu.my/id/eprint/104092/1/NUR%20FARAHANA%20BT%20DZUL%20RASHIDI%20-%20IR.pdf |