In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing
Proper wound healing is vital for the survival of higher organisms. Responses to skin injury can lead to complications such as scar formation that can affect the quality of life. In this study, keratinocytes migration (scratch assay) and zebrafish tail regeneration experiments were used to evaluate...
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| Format: | Article |
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Public Library of Science
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/101999/ |
| _version_ | 1848863688038023168 |
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| author | Chong, Wai Ting Tan, Chin Ping Cheah, Yoke Kqueen Lai, Oi Ming |
| author_facet | Chong, Wai Ting Tan, Chin Ping Cheah, Yoke Kqueen Lai, Oi Ming |
| author_sort | Chong, Wai Ting |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Proper wound healing is vital for the survival of higher organisms. Responses to skin injury can lead to complications such as scar formation that can affect the quality of life. In this study, keratinocytes migration (scratch assay) and zebrafish tail regeneration experiments were used to evaluate the wound healing effect of a tocotrienol-based nanoemulsified (NE) system against ascorbic acid and phosphate-buffered saline (PBS) as positive and negative controls, respectively. MTT assay provided a concentration range of 0.35–8.75 μg/ml of nanoemulsion that produced cell viability more than 100%. After 24 hours of treatment, the wound closure of keratinocytes were found to be significantly faster by 73.76%, 63.37% and 35.56%, respectively when treated with 3.50 μg/ml and 1.75 μg/ml of NE compared to the blank. The lethal concentration at 50% (LC50 value) obtained from acute and prolonged toxicity was almost similar, which was 4.6 mg/ml and 5.0 mg/ml, respectively. Growth of zebrafish tail regeneration treated with NE at a concentration of 2.5 mg/ml was significantly faster than the untreated zebrafish, which regenerated to 40% on the fifth day, more than 60% on the tenth day of treatment and fully recovered at the twentieth day. In conclusion, these results showed the potential of the tocotrienols-based nanoemulsified system in enhancing wound healing through accelerated wound closure. |
| first_indexed | 2025-11-15T13:36:53Z |
| format | Article |
| id | upm-101999 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:36:53Z |
| publishDate | 2022 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1019992023-09-23T00:01:44Z http://psasir.upm.edu.my/id/eprint/101999/ In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing Chong, Wai Ting Tan, Chin Ping Cheah, Yoke Kqueen Lai, Oi Ming Proper wound healing is vital for the survival of higher organisms. Responses to skin injury can lead to complications such as scar formation that can affect the quality of life. In this study, keratinocytes migration (scratch assay) and zebrafish tail regeneration experiments were used to evaluate the wound healing effect of a tocotrienol-based nanoemulsified (NE) system against ascorbic acid and phosphate-buffered saline (PBS) as positive and negative controls, respectively. MTT assay provided a concentration range of 0.35–8.75 μg/ml of nanoemulsion that produced cell viability more than 100%. After 24 hours of treatment, the wound closure of keratinocytes were found to be significantly faster by 73.76%, 63.37% and 35.56%, respectively when treated with 3.50 μg/ml and 1.75 μg/ml of NE compared to the blank. The lethal concentration at 50% (LC50 value) obtained from acute and prolonged toxicity was almost similar, which was 4.6 mg/ml and 5.0 mg/ml, respectively. Growth of zebrafish tail regeneration treated with NE at a concentration of 2.5 mg/ml was significantly faster than the untreated zebrafish, which regenerated to 40% on the fifth day, more than 60% on the tenth day of treatment and fully recovered at the twentieth day. In conclusion, these results showed the potential of the tocotrienols-based nanoemulsified system in enhancing wound healing through accelerated wound closure. Public Library of Science 2022-05-25 Article PeerReviewed Chong, Wai Ting and Tan, Chin Ping and Cheah, Yoke Kqueen and Lai, Oi Ming (2022) In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing. PLoS One, 17. pp. 1-20. ISSN 1932-6203 https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0267381 10.1371/journal.pone.0267381 |
| spellingShingle | Chong, Wai Ting Tan, Chin Ping Cheah, Yoke Kqueen Lai, Oi Ming In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing |
| title | In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing |
| title_full | In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing |
| title_fullStr | In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing |
| title_full_unstemmed | In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing |
| title_short | In-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing |
| title_sort | in-vitro and in-vivo evaluations of tocotrienol-rich nanoemulsified system on skin wound healing |
| url | http://psasir.upm.edu.my/id/eprint/101999/ http://psasir.upm.edu.my/id/eprint/101999/ http://psasir.upm.edu.my/id/eprint/101999/ |