Optimization of metabolite extraction from sago leaves tissue
Metabolic profilling have been widely studies in plants especially for highly commercial product. Currently, the study of metabolites in Metroxylon sagu becomes significantly interesting as it is one of the starch-rich sources for many potential industries. Extraction is the most critical step in...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8755/ http://ir.unimas.my/id/eprint/8755/8/Farhana%20Aktar%20ft.pdf |
| Summary: | Metabolic profilling have been widely studies in plants especially for highly commercial product. Currently,
the study of metabolites in Metroxylon sagu becomes significantly interesting as it is one of the starch-rich
sources for many potential industries. Extraction is the most critical step in metabolites profiling. This study
was conducted to optimize several parameters in the extraction method of metabolites of sago leaves. The
parameters were temperature for extraction, extraction period and extraction times which were tested using
solvent extraction method. Methanol chloroform co-solvent was used in extraction with ratio 2 : 1.The data
was measured in quantity by weighing the metabolites extracted. From factorial analysis, the result showed
there was significant correlation between temperature, extraction period and extraction time towards
metabolites extracted in leaves tissue of M. sagu. Optimal condition towards metabolites extraction was at
temperature 30oC for 2 hours at first batch extraction. |
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