Isolation of Oleaginous fungi from various sources in Kuching, sarawak
The purpose of this project was to isolate and identify oleaginous fungi from various sources in Kuching, Sarawak. Samplings of the oleaginous fungi were performed from food stalls in several markets, soil samples, sago effluent. and ftom sugar cane juices. Samplings were carried out using enrichm...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8751/ http://ir.unimas.my/id/eprint/8751/4/Paulinus%20Ngui%20Kim%20Thian%20ft.pdf |
| Summary: | The purpose of this project was to isolate and identify oleaginous fungi from various sources in Kuching, Sarawak.
Samplings of the oleaginous fungi were performed from food stalls in several markets, soil samples, sago effluent. and
ftom sugar cane juices. Samplings were carried out using enrichment medium and Rose Bengal Dichloran
Otlorampbenicol Agar. The morphologies and sizes of the selected yeaslS were observed under light microscope and
Sudan IV was used to determine the lipid accumulation abilities in the fungi cells. In addition, the oleaginous fungi
isolated from Rose Bengal Dicbloran Chloramphenicol Agar underwent Polymerase Chain Reaction and the typing of
fungi strains were identified USing partial sequencing of D1/D2 region of 265 rDNA to establish their identities. The
resultant sequcoces were compared with those available on the NCBI website database through the BlastN algorithm.
From the BiastN analysis, two yeast species; Candida tropicalis and Candida krusei were identified from the twelve
cultures. |
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