DNA isolation methods of local and imported pastry food product for halal authentication
This research project was carried out to detect porcine contaminant in pastry products using deoxyribonucleic acid (DNA) extraction and polymerase chain reaction (PCR) amplification using cyt b. Food adulteration or food fraud is an act of adding improper, cheap or unnecessary ingredient is into f...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7519/ http://ir.unimas.my/id/eprint/7519/4/Nurul%20Hidayah%20binti%20Ngadi%20ft.pdf |
| _version_ | 1848836150402220032 |
|---|---|
| author | Nurul Hidayah, Binti Ngadi. |
| author_facet | Nurul Hidayah, Binti Ngadi. |
| author_sort | Nurul Hidayah, Binti Ngadi. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | This research project was carried out to detect porcine contaminant in pastry products using deoxyribonucleic acid
(DNA) extraction and polymerase chain reaction (PCR) amplification using cyt b. Food adulteration or food fraud is
an act of adding improper, cheap or unnecessary ingredient is into food products to exploit and cheat the consumers.
Adulterations of pork meat or lard in food or processed food are very common because it is cheap and easily
available. This is a serious problem for Muslim as pork and its derivatives are haram to be consumed by Muslim.
Baking ingredients such as butter, whipping cream and cheese cream were used as samples. SUS forward and
reverse primers were utilized. Mithochondria DNA (mtDNA) is more effective to detect species-specific DNA
rather than using genomic DNA due to its different specificity expressed in the species or genera. The porcine DNA
was not successfully isolated from the samples. |
| first_indexed | 2025-11-15T06:19:11Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7519 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:11Z |
| publishDate | 2013 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-75192023-11-13T06:51:25Z http://ir.unimas.my/id/eprint/7519/ DNA isolation methods of local and imported pastry food product for halal authentication Nurul Hidayah, Binti Ngadi. Q Science (General) QH301 Biology This research project was carried out to detect porcine contaminant in pastry products using deoxyribonucleic acid (DNA) extraction and polymerase chain reaction (PCR) amplification using cyt b. Food adulteration or food fraud is an act of adding improper, cheap or unnecessary ingredient is into food products to exploit and cheat the consumers. Adulterations of pork meat or lard in food or processed food are very common because it is cheap and easily available. This is a serious problem for Muslim as pork and its derivatives are haram to be consumed by Muslim. Baking ingredients such as butter, whipping cream and cheese cream were used as samples. SUS forward and reverse primers were utilized. Mithochondria DNA (mtDNA) is more effective to detect species-specific DNA rather than using genomic DNA due to its different specificity expressed in the species or genera. The porcine DNA was not successfully isolated from the samples. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7519/4/Nurul%20Hidayah%20binti%20Ngadi%20ft.pdf Nurul Hidayah, Binti Ngadi. (2013) DNA isolation methods of local and imported pastry food product for halal authentication. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) QH301 Biology Nurul Hidayah, Binti Ngadi. DNA isolation methods of local and imported pastry food product for halal authentication |
| title | DNA isolation methods of local and imported pastry food product for halal authentication |
| title_full | DNA isolation methods of local and imported pastry food product for halal authentication |
| title_fullStr | DNA isolation methods of local and imported pastry food product for halal authentication |
| title_full_unstemmed | DNA isolation methods of local and imported pastry food product for halal authentication |
| title_short | DNA isolation methods of local and imported pastry food product for halal authentication |
| title_sort | dna isolation methods of local and imported pastry food product for halal authentication |
| topic | Q Science (General) QH301 Biology |
| url | http://ir.unimas.my/id/eprint/7519/ http://ir.unimas.my/id/eprint/7519/4/Nurul%20Hidayah%20binti%20Ngadi%20ft.pdf |