DNA isolation methods of local and imported pastry food product for halal authentication
This research project was carried out to detect porcine contaminant in pastry products using deoxyribonucleic acid (DNA) extraction and polymerase chain reaction (PCR) amplification using cyt b. Food adulteration or food fraud is an act of adding improper, cheap or unnecessary ingredient is into f...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2013
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| Online Access: | http://ir.unimas.my/id/eprint/7519/ http://ir.unimas.my/id/eprint/7519/4/Nurul%20Hidayah%20binti%20Ngadi%20ft.pdf |
| Summary: | This research project was carried out to detect porcine contaminant in pastry products using deoxyribonucleic acid
(DNA) extraction and polymerase chain reaction (PCR) amplification using cyt b. Food adulteration or food fraud is
an act of adding improper, cheap or unnecessary ingredient is into food products to exploit and cheat the consumers.
Adulterations of pork meat or lard in food or processed food are very common because it is cheap and easily
available. This is a serious problem for Muslim as pork and its derivatives are haram to be consumed by Muslim.
Baking ingredients such as butter, whipping cream and cheese cream were used as samples. SUS forward and
reverse primers were utilized. Mithochondria DNA (mtDNA) is more effective to detect species-specific DNA
rather than using genomic DNA due to its different specificity expressed in the species or genera. The porcine DNA
was not successfully isolated from the samples. |
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