Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR), SYBR Green dye, seafood
Vibrio parahaemolyticus is a foodborne pathogen and their human infection is regularly associated with the consumption of contaminated, raw or undercooked seafood. To date, many biochemical identification and confirmation are done to detect the presence of this pathogen, especially from seafood samp...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Online Access: | http://ir.unimas.my/id/eprint/7363/ http://ir.unimas.my/id/eprint/7363/3/DEVELOPMENT%20OF%20A%20SYBR%20GREEN%20BASED%20REAL-TIME%20POLYMERASE%20CHAIN%20REACTION%20ASSAY%20%28OCR%29.pdf |
| _version_ | 1848836112209936384 |
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| author | Nur Quraitu' Aini, Binti Tajudin |
| author_facet | Nur Quraitu' Aini, Binti Tajudin |
| author_sort | Nur Quraitu' Aini, Binti Tajudin |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Vibrio parahaemolyticus is a foodborne pathogen and their human infection is regularly associated with the consumption of contaminated, raw or undercooked seafood. To date, many biochemical identification and confirmation are done to detect the presence of this pathogen, especially from seafood samples. However these procedure require 2 or more days to complete. Thus, in this study we describe the development of a rapid SYBR green based real-time PCR assay for the detection and nenumeration of V.parahaemolyticus from seafood sample. The real-time PCR assay and the primers designed were highly specific, and only generated the desired amplicons with V. parahaemolyticus DNA samples against other bacteria and fungi species. Our assay was able to detect V. parahaemolyticus with high coefficient values in concentrations as low as 5.0 pg/μl DNA in pure genomic DNA solutions and 50 CFU/ml in spiked samples. |
| first_indexed | 2025-11-15T06:18:35Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7363 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:18:35Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-73632023-11-22T06:26:20Z http://ir.unimas.my/id/eprint/7363/ Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR), SYBR Green dye, seafood Nur Quraitu' Aini, Binti Tajudin GE Environmental Sciences Q Science (General) Vibrio parahaemolyticus is a foodborne pathogen and their human infection is regularly associated with the consumption of contaminated, raw or undercooked seafood. To date, many biochemical identification and confirmation are done to detect the presence of this pathogen, especially from seafood samples. However these procedure require 2 or more days to complete. Thus, in this study we describe the development of a rapid SYBR green based real-time PCR assay for the detection and nenumeration of V.parahaemolyticus from seafood sample. The real-time PCR assay and the primers designed were highly specific, and only generated the desired amplicons with V. parahaemolyticus DNA samples against other bacteria and fungi species. Our assay was able to detect V. parahaemolyticus with high coefficient values in concentrations as low as 5.0 pg/μl DNA in pure genomic DNA solutions and 50 CFU/ml in spiked samples. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7363/3/DEVELOPMENT%20OF%20A%20SYBR%20GREEN%20BASED%20REAL-TIME%20POLYMERASE%20CHAIN%20REACTION%20ASSAY%20%28OCR%29.pdf Nur Quraitu' Aini, Binti Tajudin (2012) Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR), SYBR Green dye, seafood. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences Q Science (General) Nur Quraitu' Aini, Binti Tajudin Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR), SYBR Green dye, seafood |
| title | Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood
Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR),
SYBR Green dye, seafood |
| title_full | Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood
Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR),
SYBR Green dye, seafood |
| title_fullStr | Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood
Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR),
SYBR Green dye, seafood |
| title_full_unstemmed | Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood
Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR),
SYBR Green dye, seafood |
| title_short | Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood
Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR),
SYBR Green dye, seafood |
| title_sort | development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood
key words: vibrio parahaemolyticus, real-time polymerase chain reaction (rt-pcr),
sybr green dye, seafood |
| topic | GE Environmental Sciences Q Science (General) |
| url | http://ir.unimas.my/id/eprint/7363/ http://ir.unimas.my/id/eprint/7363/3/DEVELOPMENT%20OF%20A%20SYBR%20GREEN%20BASED%20REAL-TIME%20POLYMERASE%20CHAIN%20REACTION%20ASSAY%20%28OCR%29.pdf |