Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques
Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana geno...
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| Format: | Article |
| Language: | English |
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Prof. H.S. Srivastava Foundation for Science and Society 2014
2014
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| Online Access: | http://ir.unimas.my/id/eprint/5195/ http://ir.unimas.my/id/eprint/5195/1/Kit-Siong%20Liew.pdf |
| _version_ | 1848835605833711616 |
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| author | Kit-Siong, Liew Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah |
| author_facet | Kit-Siong, Liew Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah |
| author_sort | Kit-Siong, Liew |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana genome using ISSR-suppression PCR techniques. The number of alleles and PIC values ranged from 3 to 8 alleles per locus and from 0.488 to 0.792, respectively. Three microsatellite loci were deviated from Hardy-Weinberg equilibrium (P<0.05). The transferability rate ranged from 24 to 100 % among the three indigenous tree species tested. This indicates that the newly developed microsatellite markers would be useful tools for population genetic studies on D. moluccana and other indigenous tree species. |
| first_indexed | 2025-11-15T06:10:32Z |
| format | Article |
| id | unimas-5195 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:10:32Z |
| publishDate | 2014 |
| publisher | Prof. H.S. Srivastava Foundation for Science and Society 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-51952022-01-26T01:38:31Z http://ir.unimas.my/id/eprint/5195/ Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques Kit-Siong, Liew Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah QR Microbiology SB Plant culture Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana genome using ISSR-suppression PCR techniques. The number of alleles and PIC values ranged from 3 to 8 alleles per locus and from 0.488 to 0.792, respectively. Three microsatellite loci were deviated from Hardy-Weinberg equilibrium (P<0.05). The transferability rate ranged from 24 to 100 % among the three indigenous tree species tested. This indicates that the newly developed microsatellite markers would be useful tools for population genetic studies on D. moluccana and other indigenous tree species. Prof. H.S. Srivastava Foundation for Science and Society 2014 2014 Article PeerReviewed text en http://ir.unimas.my/id/eprint/5195/1/Kit-Siong%20Liew.pdf Kit-Siong, Liew and Wei-Seng, Ho and Shek-Ling, Pang and Julaihi, Abdullah (2014) Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. Physiol Mol Biol Plants. http://www.ncbi.nlm.nih.gov/ |
| spellingShingle | QR Microbiology SB Plant culture Kit-Siong, Liew Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques |
| title | Development and characterization of microsatellite markers
in sawih tree (Duabanga moluccana Blume) using
ISSR-suppression PCR techniques |
| title_full | Development and characterization of microsatellite markers
in sawih tree (Duabanga moluccana Blume) using
ISSR-suppression PCR techniques |
| title_fullStr | Development and characterization of microsatellite markers
in sawih tree (Duabanga moluccana Blume) using
ISSR-suppression PCR techniques |
| title_full_unstemmed | Development and characterization of microsatellite markers
in sawih tree (Duabanga moluccana Blume) using
ISSR-suppression PCR techniques |
| title_short | Development and characterization of microsatellite markers
in sawih tree (Duabanga moluccana Blume) using
ISSR-suppression PCR techniques |
| title_sort | development and characterization of microsatellite markers
in sawih tree (duabanga moluccana blume) using
issr-suppression pcr techniques |
| topic | QR Microbiology SB Plant culture |
| url | http://ir.unimas.my/id/eprint/5195/ http://ir.unimas.my/id/eprint/5195/ http://ir.unimas.my/id/eprint/5195/1/Kit-Siong%20Liew.pdf |