Adaptation of in vitro cytoadherence assay to Plasmodium knowlesi field isolates
P. knowlesi was the first Plasmodium species in which antigenic variation was observed. Variation was due to schizont infected cell agglutination (SICAvar) antigens expressed by the parasite and transported to the exposed surface of the host erythrocyte [1]. PfEMP1 is P. falciparum’s orthologue...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BioMed Central Ltd.
2010
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15805/ http://ir.unimas.my/id/eprint/15805/1/Adaptation%20of%20in%20vitro%20cytoadherence%20assay%20%28abstract%29.pdf |
| Summary: | P. knowlesi was the first Plasmodium species in which
antigenic variation was observed. Variation was due to
schizont infected cell agglutination (SICAvar) antigens
expressed by the parasite and transported to the
exposed surface of the host erythrocyte [1]. PfEMP1 is
P. falciparum’s orthologue of P. knowlesi’s SICA proteins
[2]. In P. falciparum PfEMP1 is associated with infected
erythrocytes binding to receptors such as ICAM-1
expressed on the endothelial cells of the host microvasculature.
Here, we use a static protein assay [3] to determine
if naturally occurring human P. knowlesi infections
can cause erythrocytes to bind to ICAM-1, VCAM-1
and CD36. |
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