Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation
Amylase is recognized as one of the important commercial enzymes. This group of enzymes has the ability in hydrolyzing starch into smaller oligosacharides. The present work aimed to determine the optimum fermentation conditions for maximum production of crude amylase enzyme by Aspergillus flavus NSH...
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SciELO
2016
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| Online Access: | http://ir.unimas.my/id/eprint/13583/ http://ir.unimas.my/id/eprint/13583/7/Application%20of%20Response%20Surface%20methodology%20%28abstract%29.pdf |
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| author | Siti Ratina, Mustafa Ahmad, Husaini Cirilo Nolasco, Hipolito Hasnain, Hussain Nurshikin, Suhaili Hairul Azman, Roslan |
| author_facet | Siti Ratina, Mustafa Ahmad, Husaini Cirilo Nolasco, Hipolito Hasnain, Hussain Nurshikin, Suhaili Hairul Azman, Roslan |
| author_sort | Siti Ratina, Mustafa |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Amylase is recognized as one of the important commercial enzymes. This group of enzymes has the ability in hydrolyzing starch into smaller oligosacharides. The present work aimed to determine the optimum fermentation conditions for maximum production of crude amylase enzyme by Aspergillus flavus NSH9 employing response surface methodology (RSM).Central composite design (CCD) was applied to determine the optimal fermentation condition with respect to the four main process parameters such as temperature, initial moisture content, pH and the incubation period. Solid state fermentation (SSF) was performed using 5.0 g of sago hampas inoculated with 1x107sporesmL-1following the experimental design obtained using CCD and further optimized by RSM. The initial moisture, pH and temperature showed significant effect on the amylase production (p<0.05). The maximum amylase activity produced was achieved and recorded as 1.055 ± 0.03U mL-1after four days of fermentation period with 100% (v/v) moisture holding capacity, pH 6.5 and temperature at 28°C. The optimum fermentation conditions for amylase production was determined with A. flavusNSH9 on sago hampas. |
| first_indexed | 2025-11-15T06:39:39Z |
| format | Article |
| id | unimas-13583 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:39:39Z |
| publishDate | 2016 |
| publisher | SciELO |
| recordtype | eprints |
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| spelling | unimas-135832016-09-25T20:10:22Z http://ir.unimas.my/id/eprint/13583/ Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation Siti Ratina, Mustafa Ahmad, Husaini Cirilo Nolasco, Hipolito Hasnain, Hussain Nurshikin, Suhaili Hairul Azman, Roslan GE Environmental Sciences S Agriculture (General) Amylase is recognized as one of the important commercial enzymes. This group of enzymes has the ability in hydrolyzing starch into smaller oligosacharides. The present work aimed to determine the optimum fermentation conditions for maximum production of crude amylase enzyme by Aspergillus flavus NSH9 employing response surface methodology (RSM).Central composite design (CCD) was applied to determine the optimal fermentation condition with respect to the four main process parameters such as temperature, initial moisture content, pH and the incubation period. Solid state fermentation (SSF) was performed using 5.0 g of sago hampas inoculated with 1x107sporesmL-1following the experimental design obtained using CCD and further optimized by RSM. The initial moisture, pH and temperature showed significant effect on the amylase production (p<0.05). The maximum amylase activity produced was achieved and recorded as 1.055 ± 0.03U mL-1after four days of fermentation period with 100% (v/v) moisture holding capacity, pH 6.5 and temperature at 28°C. The optimum fermentation conditions for amylase production was determined with A. flavusNSH9 on sago hampas. SciELO 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/13583/7/Application%20of%20Response%20Surface%20methodology%20%28abstract%29.pdf Siti Ratina, Mustafa and Ahmad, Husaini and Cirilo Nolasco, Hipolito and Hasnain, Hussain and Nurshikin, Suhaili and Hairul Azman, Roslan (2016) Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation. Biological and Applied Sciences, 59. pp. 1-10. ISSN 1678-4324 http://dx.doi.org/10.1590/1678-4324-2016150632 http://dx.doi.org/10.1590/1678-4324-2016150632 |
| spellingShingle | GE Environmental Sciences S Agriculture (General) Siti Ratina, Mustafa Ahmad, Husaini Cirilo Nolasco, Hipolito Hasnain, Hussain Nurshikin, Suhaili Hairul Azman, Roslan Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation |
| title | Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation |
| title_full | Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation |
| title_fullStr | Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation |
| title_full_unstemmed | Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation |
| title_short | Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation |
| title_sort | application of response surface methodology for optimizing process parameters in the production of amylase by aspergillus flavusnsh9 under solid state fermentation |
| topic | GE Environmental Sciences S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/13583/ http://ir.unimas.my/id/eprint/13583/ http://ir.unimas.my/id/eprint/13583/ http://ir.unimas.my/id/eprint/13583/7/Application%20of%20Response%20Surface%20methodology%20%28abstract%29.pdf |