Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL...
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| Format: | Article |
| Language: | English |
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Trans Tech Publications
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/30520/ http://umpir.ump.edu.my/id/eprint/30520/1/12.1%20Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis.pdf |
| _version_ | 1848823535080833024 |
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| author | Nur Husnina Iffah, Bakar Thong, Chuan Lee Mohd Hairul, Ab. Rahim Raha, Ahmad Raus Dayang Fredalina, Basri Noor Suhana, Adzahar |
| author_facet | Nur Husnina Iffah, Bakar Thong, Chuan Lee Mohd Hairul, Ab. Rahim Raha, Ahmad Raus Dayang Fredalina, Basri Noor Suhana, Adzahar |
| author_sort | Nur Husnina Iffah, Bakar |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract. |
| first_indexed | 2025-11-15T02:58:40Z |
| format | Article |
| id | ump-30520 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:58:40Z |
| publishDate | 2020 |
| publisher | Trans Tech Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-305202021-02-08T03:45:40Z http://umpir.ump.edu.my/id/eprint/30520/ Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis Nur Husnina Iffah, Bakar Thong, Chuan Lee Mohd Hairul, Ab. Rahim Raha, Ahmad Raus Dayang Fredalina, Basri Noor Suhana, Adzahar T Technology (General) A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract. Trans Tech Publications 2020 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/30520/1/12.1%20Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis.pdf Nur Husnina Iffah, Bakar and Thong, Chuan Lee and Mohd Hairul, Ab. Rahim and Raha, Ahmad Raus and Dayang Fredalina, Basri and Noor Suhana, Adzahar (2020) Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis. Materials Science Forum, 981. pp. 265-270. ISSN 0255-5476. (Published) https://doi.org/10.4028/www.scientific.net/MSF.981.265 https://doi.org/10.4028/www.scientific.net/MSF.981.265 |
| spellingShingle | T Technology (General) Nur Husnina Iffah, Bakar Thong, Chuan Lee Mohd Hairul, Ab. Rahim Raha, Ahmad Raus Dayang Fredalina, Basri Noor Suhana, Adzahar Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_full | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_fullStr | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_full_unstemmed | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_short | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_sort | real-time cytotoxicity assay of water extracts hibiscus rosa-sinensis |
| topic | T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/30520/ http://umpir.ump.edu.my/id/eprint/30520/ http://umpir.ump.edu.my/id/eprint/30520/ http://umpir.ump.edu.my/id/eprint/30520/1/12.1%20Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis.pdf |