Comparative evaluation of protease extraction from leaves of syzygium polyanthum
In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogenbond acceptor (HBA) solvent based extraction and Trichloro...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Gadjah Mada University
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/25336/ http://umpir.ump.edu.my/id/eprint/25336/1/Comparative%20evaluation%20of%20protease%20extraction%20from%20leaves.pdf |
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| author | Noorhafiza, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani Mohd Hairul, Ab. Rahim |
| author_facet | Noorhafiza, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani Mohd Hairul, Ab. Rahim |
| author_sort | Noorhafiza, Yahya |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogenbond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods. |
| first_indexed | 2025-11-15T02:38:20Z |
| format | Article |
| id | ump-25336 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:38:20Z |
| publishDate | 2019 |
| publisher | Gadjah Mada University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-253362019-10-25T03:32:28Z http://umpir.ump.edu.my/id/eprint/25336/ Comparative evaluation of protease extraction from leaves of syzygium polyanthum Noorhafiza, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani Mohd Hairul, Ab. Rahim QD Chemistry QP Physiology TP Chemical technology In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogenbond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods. Gadjah Mada University 2019-05 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/25336/1/Comparative%20evaluation%20of%20protease%20extraction%20from%20leaves.pdf Noorhafiza, Yahya and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Rohaida, Che Man and Shalyda, Md Shaarani and Mohd Hairul, Ab. Rahim (2019) Comparative evaluation of protease extraction from leaves of syzygium polyanthum. Indonesian Journal of Chemistry, 19 (2). pp. 503-510. ISSN 1411-9420 (Print); 2460-1578 (Online). (Published) https://doi.org/10.22146/ijc.40860 https://doi.org/10.22146/ijc.40860 |
| spellingShingle | QD Chemistry QP Physiology TP Chemical technology Noorhafiza, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani Mohd Hairul, Ab. Rahim Comparative evaluation of protease extraction from leaves of syzygium polyanthum |
| title | Comparative evaluation of protease extraction from leaves of syzygium polyanthum |
| title_full | Comparative evaluation of protease extraction from leaves of syzygium polyanthum |
| title_fullStr | Comparative evaluation of protease extraction from leaves of syzygium polyanthum |
| title_full_unstemmed | Comparative evaluation of protease extraction from leaves of syzygium polyanthum |
| title_short | Comparative evaluation of protease extraction from leaves of syzygium polyanthum |
| title_sort | comparative evaluation of protease extraction from leaves of syzygium polyanthum |
| topic | QD Chemistry QP Physiology TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/25336/ http://umpir.ump.edu.my/id/eprint/25336/ http://umpir.ump.edu.my/id/eprint/25336/ http://umpir.ump.edu.my/id/eprint/25336/1/Comparative%20evaluation%20of%20protease%20extraction%20from%20leaves.pdf |