Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots
In the current study, we investigated the chemical composition, in vitro antioxidant, antimicrobial and haemolytic activities of the crude (methanolic) and n-hexane, chloroform, and ethyl acetate extracts of roots of Crambe cordifolia Steven. The GC-MS analysis led to identification of erucic acid (...
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| Format: | Article |
| Language: | English |
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Romanian Society for Pharmaceutical Sciences
2018
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| Online Access: | http://umpir.ump.edu.my/id/eprint/23930/ http://umpir.ump.edu.my/id/eprint/23930/1/Chemical%20composition%20and%20antioxidant.pdf |
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| author | Rashid, Muhammad Abid Akhtar, Muhammad Nadeem Ashraf, Aisha Nazir, Sadia Ijaz, Anum Noradila, Abdullah Omar Noor, Sadia Basit, Abdul Tareen, Rasool Bakhsh |
| author_facet | Rashid, Muhammad Abid Akhtar, Muhammad Nadeem Ashraf, Aisha Nazir, Sadia Ijaz, Anum Noradila, Abdullah Omar Noor, Sadia Basit, Abdul Tareen, Rasool Bakhsh |
| author_sort | Rashid, Muhammad Abid |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | In the current study, we investigated the chemical composition, in vitro antioxidant, antimicrobial and haemolytic activities of the crude (methanolic) and n-hexane, chloroform, and ethyl acetate extracts of roots of Crambe cordifolia Steven. The GC-MS analysis led to identification of erucic acid (20.8%), p-cymene (20.9%), palmitic acid (19.2%) and vitamin E (13.8%) as major components of methanol, n-hexane, chloroform and ethyl acetate extracts respectively. The results of spectrophotometric assays showed that the methanol extract had the highest total phenolic contents (210.3 ± 0.74 μg
GAE/mg of dry plant material), total flavonoid contents (75.5 ± 0.32 μg QE/mg of dry plant material) and antioxidant
potential. It was established by disc diffusion and minimum inhibitory concentration methods that, overall, the methanol
extract exhibited the highest antimicrobial activity against most of the tested microbial strains (Escherichia coli, Bacillus
subtilis, Pasteurella multocida, Staphylococcus aureus, Aspergillus niger and Fusarium solani) as compared with the nhexane, chloroform, and ethyl acetate extracts. The outcome of the haemolytic assay showed that the tested extract and
different solvent fractions were safe to human erythrocyte membrane |
| first_indexed | 2025-11-15T02:33:01Z |
| format | Article |
| id | ump-23930 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:33:01Z |
| publishDate | 2018 |
| publisher | Romanian Society for Pharmaceutical Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-239302019-01-23T08:02:18Z http://umpir.ump.edu.my/id/eprint/23930/ Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots Rashid, Muhammad Abid Akhtar, Muhammad Nadeem Ashraf, Aisha Nazir, Sadia Ijaz, Anum Noradila, Abdullah Omar Noor, Sadia Basit, Abdul Tareen, Rasool Bakhsh QR Microbiology In the current study, we investigated the chemical composition, in vitro antioxidant, antimicrobial and haemolytic activities of the crude (methanolic) and n-hexane, chloroform, and ethyl acetate extracts of roots of Crambe cordifolia Steven. The GC-MS analysis led to identification of erucic acid (20.8%), p-cymene (20.9%), palmitic acid (19.2%) and vitamin E (13.8%) as major components of methanol, n-hexane, chloroform and ethyl acetate extracts respectively. The results of spectrophotometric assays showed that the methanol extract had the highest total phenolic contents (210.3 ± 0.74 μg GAE/mg of dry plant material), total flavonoid contents (75.5 ± 0.32 μg QE/mg of dry plant material) and antioxidant potential. It was established by disc diffusion and minimum inhibitory concentration methods that, overall, the methanol extract exhibited the highest antimicrobial activity against most of the tested microbial strains (Escherichia coli, Bacillus subtilis, Pasteurella multocida, Staphylococcus aureus, Aspergillus niger and Fusarium solani) as compared with the nhexane, chloroform, and ethyl acetate extracts. The outcome of the haemolytic assay showed that the tested extract and different solvent fractions were safe to human erythrocyte membrane Romanian Society for Pharmaceutical Sciences 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23930/1/Chemical%20composition%20and%20antioxidant.pdf Rashid, Muhammad Abid and Akhtar, Muhammad Nadeem and Ashraf, Aisha and Nazir, Sadia and Ijaz, Anum and Noradila, Abdullah Omar and Noor, Sadia and Basit, Abdul and Tareen, Rasool Bakhsh (2018) Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots. Farmacia, 66 (1). pp. 165-171. ISSN 2065-0019. (Published) http://www.revistafarmacia.ro/201801/art-21-Muhammad_Rashid_165-171.pdf |
| spellingShingle | QR Microbiology Rashid, Muhammad Abid Akhtar, Muhammad Nadeem Ashraf, Aisha Nazir, Sadia Ijaz, Anum Noradila, Abdullah Omar Noor, Sadia Basit, Abdul Tareen, Rasool Bakhsh Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots |
| title | Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots |
| title_full | Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots |
| title_fullStr | Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots |
| title_full_unstemmed | Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots |
| title_short | Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots |
| title_sort | chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots |
| topic | QR Microbiology |
| url | http://umpir.ump.edu.my/id/eprint/23930/ http://umpir.ump.edu.my/id/eprint/23930/ http://umpir.ump.edu.my/id/eprint/23930/1/Chemical%20composition%20and%20antioxidant.pdf |