Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia

The extracts from Vernonia amygdalina leaves dried using different modes of drying were examined for their antioxidant activities. The component identification was carried out on extract with the highest antioxidant property using GC–MS analysis. Using DPPH, ABTS+ and H2O2 radical scavenging assays,...

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Main Authors: Alara, Oluwaseun Ruth, Nour, A. H., Siti Kholijah, Abdul Mudalip, Olalere, Olusegun Abayomi
Format: Article
Language:English
Published: Elsevier Ltd 2018
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/23755/
http://umpir.ump.edu.my/id/eprint/23755/1/Effect%20of%20drying%20methods%20on%20the%20free%20radicals.pdf
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author Alara, Oluwaseun Ruth
Nour, A. H.
Siti Kholijah, Abdul Mudalip
Olalere, Olusegun Abayomi
author_facet Alara, Oluwaseun Ruth
Nour, A. H.
Siti Kholijah, Abdul Mudalip
Olalere, Olusegun Abayomi
author_sort Alara, Oluwaseun Ruth
building UMP Institutional Repository
collection Online Access
description The extracts from Vernonia amygdalina leaves dried using different modes of drying were examined for their antioxidant activities. The component identification was carried out on extract with the highest antioxidant property using GC–MS analysis. Using DPPH, ABTS+ and H2O2 radical scavenging assays, the shade, the sun, oven at 40, 50, and 60 °C dried sample extracts were screened. The obtained scavenging activities were in the order: shade drying > sun drying > oven drying at 40 °C > oven drying at 50 °C > oven drying at 60 °C. The extract from shade-dried sample exhibited the highest activity by scavenging 63.21–92.25% of DPPH, 75.32–93.15% of ABTS+, and 82.53–95.78% of H2O2 radicals, which were higher than those of ascorbic and gallic acid scavenging activities. In addition, the effects of drying methods and extract concentrations on the antioxidant activity of V. amygdalina leaf were significant (p < 0.05). A total number of 11 major compounds were identified from the shade-dried extract viz: phytol (43.69%), (z,z,z)-methyl ester-9,12,15-octadecatrienoic acid (17.60%), 2-methyl-3-hexanol (6.83%), ethyl ester-9,12-octadecadienoic acid (6.07%), ethyl ester linoleic acid (5.19%), ethyl ester hexadecanoic acid (5.06), heneicosane (4.15%), heptacosane (3.25%), and others are less than 3%. Therefore, it can be deduced that an increase in drying temperature may deteriorate the antioxidant activity of Vernonia amygdalina leaf.
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spelling ump-237552019-09-10T04:03:31Z http://umpir.ump.edu.my/id/eprint/23755/ Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi TP Chemical technology The extracts from Vernonia amygdalina leaves dried using different modes of drying were examined for their antioxidant activities. The component identification was carried out on extract with the highest antioxidant property using GC–MS analysis. Using DPPH, ABTS+ and H2O2 radical scavenging assays, the shade, the sun, oven at 40, 50, and 60 °C dried sample extracts were screened. The obtained scavenging activities were in the order: shade drying > sun drying > oven drying at 40 °C > oven drying at 50 °C > oven drying at 60 °C. The extract from shade-dried sample exhibited the highest activity by scavenging 63.21–92.25% of DPPH, 75.32–93.15% of ABTS+, and 82.53–95.78% of H2O2 radicals, which were higher than those of ascorbic and gallic acid scavenging activities. In addition, the effects of drying methods and extract concentrations on the antioxidant activity of V. amygdalina leaf were significant (p < 0.05). A total number of 11 major compounds were identified from the shade-dried extract viz: phytol (43.69%), (z,z,z)-methyl ester-9,12,15-octadecatrienoic acid (17.60%), 2-methyl-3-hexanol (6.83%), ethyl ester-9,12-octadecadienoic acid (6.07%), ethyl ester linoleic acid (5.19%), ethyl ester hexadecanoic acid (5.06), heneicosane (4.15%), heptacosane (3.25%), and others are less than 3%. Therefore, it can be deduced that an increase in drying temperature may deteriorate the antioxidant activity of Vernonia amygdalina leaf. Elsevier Ltd 2018 Article NonPeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/23755/1/Effect%20of%20drying%20methods%20on%20the%20free%20radicals.pdf Alara, Oluwaseun Ruth and Nour, A. H. and Siti Kholijah, Abdul Mudalip and Olalere, Olusegun Abayomi (2018) Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia. Journal of King Saud University - Science. pp. 1-5. ISSN 1018-3647. (In Press / Online First) (In Press / Online First) https://doi.org/10.1016/j.jksus.2017.05.018 https://doi.org/10.1016/j.jksus.2017.05.018
spellingShingle TP Chemical technology
Alara, Oluwaseun Ruth
Nour, A. H.
Siti Kholijah, Abdul Mudalip
Olalere, Olusegun Abayomi
Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_full Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_fullStr Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_full_unstemmed Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_short Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_sort effect of drying methods on the free radicals scavenging activity of vernonia amygdalina growing in malaysia
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/23755/
http://umpir.ump.edu.my/id/eprint/23755/
http://umpir.ump.edu.my/id/eprint/23755/
http://umpir.ump.edu.my/id/eprint/23755/1/Effect%20of%20drying%20methods%20on%20the%20free%20radicals.pdf