Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White
Membrane chromatography, which uses an adsorptive membrane, is an alternative technology that overcomes the limitations of packed-bed column chromatography. Mixed matrix membrane (MMM) preparation is a simple method by which adsorptive membranes can be prepared. In the current study, several potenti...
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| Format: | Article |
| Language: | English |
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Springer
2016
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| Online Access: | http://umpir.ump.edu.my/id/eprint/11899/ http://umpir.ump.edu.my/id/eprint/11899/1/Adsorptive%20Cation%20Exchanger%20Mixed%20Matrix%20Membrane%20Chromatography%20for%20the%20Isolation%20of%20Lysozyme%20from%20Chicken%20Egg%20White.pdf |
| _version_ | 1848819100940238848 |
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| author | N., Shamsinar Syed Mohd Saufi, Tuan Chik |
| author_facet | N., Shamsinar Syed Mohd Saufi, Tuan Chik |
| author_sort | N., Shamsinar |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Membrane chromatography, which uses an adsorptive membrane, is an alternative technology that overcomes the limitations of packed-bed column chromatography. Mixed matrix membrane (MMM) preparation is a simple method by which adsorptive membranes can be prepared. In the current study, several potential low-cost cation exchange (CEX) resins were screened for their ability to bind lysozyme (LYS) at various pH values prior to the preparation of CEX MMMs. The best binding was shown by Lewatit CNP105 at pH 7. A subsequently prepared Lewatit CNP105—CEX MMM followed the Langmuir adsorption isotherm, with a maximum binding capacity of 223 mg LYS/g membrane. In batch binding from a chicken egg white (CEW) solution at pH 7, both positively charged (LYS, pI 10.7) and negatively charged (conalbumin (CNL), pI 6.1; and ovalbumin (OVL), pI 4.5) proteins were bound onto the MMM. However, CNL and OVL were only loosely bound and were washed out during the washing step. Almost pure LYS was recovered during the elution step, as shown by SDS–PAGE gel analysis. |
| first_indexed | 2025-11-15T01:48:12Z |
| format | Article |
| id | ump-11899 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:48:12Z |
| publishDate | 2016 |
| publisher | Springer |
| recordtype | eprints |
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| spelling | ump-118992018-09-06T07:22:19Z http://umpir.ump.edu.my/id/eprint/11899/ Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White N., Shamsinar Syed Mohd Saufi, Tuan Chik TP Chemical technology Membrane chromatography, which uses an adsorptive membrane, is an alternative technology that overcomes the limitations of packed-bed column chromatography. Mixed matrix membrane (MMM) preparation is a simple method by which adsorptive membranes can be prepared. In the current study, several potential low-cost cation exchange (CEX) resins were screened for their ability to bind lysozyme (LYS) at various pH values prior to the preparation of CEX MMMs. The best binding was shown by Lewatit CNP105 at pH 7. A subsequently prepared Lewatit CNP105—CEX MMM followed the Langmuir adsorption isotherm, with a maximum binding capacity of 223 mg LYS/g membrane. In batch binding from a chicken egg white (CEW) solution at pH 7, both positively charged (LYS, pI 10.7) and negatively charged (conalbumin (CNL), pI 6.1; and ovalbumin (OVL), pI 4.5) proteins were bound onto the MMM. However, CNL and OVL were only loosely bound and were washed out during the washing step. Almost pure LYS was recovered during the elution step, as shown by SDS–PAGE gel analysis. Springer 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11899/1/Adsorptive%20Cation%20Exchanger%20Mixed%20Matrix%20Membrane%20Chromatography%20for%20the%20Isolation%20of%20Lysozyme%20from%20Chicken%20Egg%20White.pdf N., Shamsinar and Syed Mohd Saufi, Tuan Chik (2016) Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White. Arabian Journal for Science and Engineering. pp. 1-7. ISSN 1319-8025 (print); 2191-4281 (online). (Published) http://dx.doi.org/10.1007/s13369-015-1939-8 DOI: 10.1007/s13369-015-1939-8 |
| spellingShingle | TP Chemical technology N., Shamsinar Syed Mohd Saufi, Tuan Chik Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White |
| title | Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White |
| title_full | Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White |
| title_fullStr | Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White |
| title_full_unstemmed | Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White |
| title_short | Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White |
| title_sort | adsorptive cation exchanger mixed matrix membrane chromatography for the isolation of lysozyme from chicken egg white |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/11899/ http://umpir.ump.edu.my/id/eprint/11899/ http://umpir.ump.edu.my/id/eprint/11899/ http://umpir.ump.edu.my/id/eprint/11899/1/Adsorptive%20Cation%20Exchanger%20Mixed%20Matrix%20Membrane%20Chromatography%20for%20the%20Isolation%20of%20Lysozyme%20from%20Chicken%20Egg%20White.pdf |