Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui
Background: Antibodies to citrullinated proteins are widely used as diagnostic marker for rheumatoid arthritis (RA). Antibodies against carbamylated proteins were also suggested to be used as a potential biomarker for RA. It has been speculated that citrullination and carbamylation occur in infla...
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| Format: | Thesis |
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2019
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| Online Access: | http://studentsrepo.um.edu.my/11224/ http://studentsrepo.um.edu.my/11224/4/yin_hui.pdf |
| _version_ | 1848774328507695104 |
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| author | Lee, Yin Hui |
| author_facet | Lee, Yin Hui |
| author_sort | Lee, Yin Hui |
| building | UM Research Repository |
| collection | Online Access |
| description | Background: Antibodies to citrullinated proteins are widely used as diagnostic marker
for rheumatoid arthritis (RA). Antibodies against carbamylated proteins were also
suggested to be used as a potential biomarker for RA. It has been speculated that
citrullination and carbamylation occur in inflamed periodontium which can be a source
of autoantibodies to induce immune responses resulting in the development and
progression of RA. Aim: To determine the presence and location of citrullinated and
carbamylated proteins in the gingival tissues and compare their abundance in
periodontitis (PD) participants with and without RA. Materials and Methods: Gingival
tissue samples of healthy control (n = 5), PD with RA (n = 5) and PD without RA (n = 5)
were collected. Specimens were formalin-fixed, paraffin embedded and sectioned at 4
µm. The tissue sections were analysed for the presence of citrullinated and
carbamylated proteins by immunohistochemistry. Semi-quantitative analysis was
performed to quantify and compare the protein abundance between groups. Results:
Number of cells containing citrullinated and carbamylated proteins with higher intensity
was markedly increased in gingival tissues from PD with or without RA in comparison
with healthy controls. Conclusion: Inflamed gingival tissue is a potential extra-articular
source of citrullinated and carbamylated proteins. The extent to which these proteins
contribute to the pathogenesis of RA warrants further elucidation. |
| first_indexed | 2025-11-14T13:56:33Z |
| format | Thesis |
| id | um-11224 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T13:56:33Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-112242022-01-02T17:08:38Z Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui Lee, Yin Hui RK Dentistry Background: Antibodies to citrullinated proteins are widely used as diagnostic marker for rheumatoid arthritis (RA). Antibodies against carbamylated proteins were also suggested to be used as a potential biomarker for RA. It has been speculated that citrullination and carbamylation occur in inflamed periodontium which can be a source of autoantibodies to induce immune responses resulting in the development and progression of RA. Aim: To determine the presence and location of citrullinated and carbamylated proteins in the gingival tissues and compare their abundance in periodontitis (PD) participants with and without RA. Materials and Methods: Gingival tissue samples of healthy control (n = 5), PD with RA (n = 5) and PD without RA (n = 5) were collected. Specimens were formalin-fixed, paraffin embedded and sectioned at 4 µm. The tissue sections were analysed for the presence of citrullinated and carbamylated proteins by immunohistochemistry. Semi-quantitative analysis was performed to quantify and compare the protein abundance between groups. Results: Number of cells containing citrullinated and carbamylated proteins with higher intensity was markedly increased in gingival tissues from PD with or without RA in comparison with healthy controls. Conclusion: Inflamed gingival tissue is a potential extra-articular source of citrullinated and carbamylated proteins. The extent to which these proteins contribute to the pathogenesis of RA warrants further elucidation. 2019 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/11224/4/yin_hui.pdf Lee, Yin Hui (2019) Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui. Masters thesis, Universiti Malaya. http://studentsrepo.um.edu.my/11224/ |
| spellingShingle | RK Dentistry Lee, Yin Hui Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui |
| title | Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui |
| title_full | Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui |
| title_fullStr | Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui |
| title_full_unstemmed | Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui |
| title_short | Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui |
| title_sort | localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / lee yin hui |
| topic | RK Dentistry |
| url | http://studentsrepo.um.edu.my/11224/ http://studentsrepo.um.edu.my/11224/4/yin_hui.pdf |