Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek
Silicon, glass, and ceramic are commonly used base substrates for gold (Au) deposition on label-free DNA biosensors. This study aimed to investigate the suitability of applying flame retardant 4 (FR4) as a base substrate and polydimethylsiloxane (PDMS) as a coating substrate. A thermal evaporator wa...
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| Format: | Article |
| Language: | English |
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Universiti Teknologi MARA, Pulau Pinang & Pusat Penerbitan Universiti (UPENA)
2013
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/8898/ |
| _version_ | 1848802904968790016 |
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| author | Noorsal, Emilia Ibrahim, Mohammad Nizam Abd Manaf, Asrulnizam |
| author_facet | Noorsal, Emilia Ibrahim, Mohammad Nizam Abd Manaf, Asrulnizam |
| author_sort | Noorsal, Emilia |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Silicon, glass, and ceramic are commonly used base substrates for gold (Au) deposition on label-free DNA biosensors. This study aimed to investigate the suitability of applying flame retardant 4 (FR4) as a base substrate and polydimethylsiloxane (PDMS) as a coating substrate. A thermal evaporator was used to fabricate copper/nickel/Au metals and ensure even deposition of Au throughout FR4. UV exposure soft lithography and wet etching were applied to develop a three-electrode pattern on the metals fabricated on FR4. A simple and straightforward technique on PDMS–FR4 bonding was proved to work at the shear strength of 55 kPa. A cyclic voltammetry (CV) method was performed using the fabricated FR4 sensor to analyze bare Au, DNA immobilization and hybridization. The FR4-based Au fabricated with PDMS acted as a coating layer between terminals and sensing electrodes, and can thus be used as a label-free DNA sensor. |
| first_indexed | 2025-11-14T21:30:46Z |
| format | Article |
| id | uitm-8898 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:30:46Z |
| publishDate | 2013 |
| publisher | Universiti Teknologi MARA, Pulau Pinang & Pusat Penerbitan Universiti (UPENA) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-88982019-03-18T07:20:53Z https://ir.uitm.edu.my/id/eprint/8898/ Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek esteem Noorsal, Emilia Ibrahim, Mohammad Nizam Abd Manaf, Asrulnizam TK Electrical engineering. Electronics. Nuclear engineering Silicon, glass, and ceramic are commonly used base substrates for gold (Au) deposition on label-free DNA biosensors. This study aimed to investigate the suitability of applying flame retardant 4 (FR4) as a base substrate and polydimethylsiloxane (PDMS) as a coating substrate. A thermal evaporator was used to fabricate copper/nickel/Au metals and ensure even deposition of Au throughout FR4. UV exposure soft lithography and wet etching were applied to develop a three-electrode pattern on the metals fabricated on FR4. A simple and straightforward technique on PDMS–FR4 bonding was proved to work at the shear strength of 55 kPa. A cyclic voltammetry (CV) method was performed using the fabricated FR4 sensor to analyze bare Au, DNA immobilization and hybridization. The FR4-based Au fabricated with PDMS acted as a coating layer between terminals and sensing electrodes, and can thus be used as a label-free DNA sensor. Universiti Teknologi MARA, Pulau Pinang & Pusat Penerbitan Universiti (UPENA) 2013 Article NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/8898/1/AJ_IRNI%20HAMIZA%20HAMZAH%20EAJ%2013.pdf Noorsal, Emilia and Ibrahim, Mohammad Nizam and Abd Manaf, Asrulnizam (2013) Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek. (2013) Esteem Academic Journal <https://ir.uitm.edu.my/view/publication/Esteem_Academic_Journal.html>, 9 (1). pp. 1-11. ISSN 1675-7939 |
| spellingShingle | TK Electrical engineering. Electronics. Nuclear engineering Noorsal, Emilia Ibrahim, Mohammad Nizam Abd Manaf, Asrulnizam Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek |
| title | Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek |
| title_full | Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek |
| title_fullStr | Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek |
| title_full_unstemmed | Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek |
| title_short | Fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free DNA sensor / Irni Hamiza Hamzah, Emilia Noorsal, Mohammad Nizam Ibrahim, Asrulnizam Abd Manaf and Othman Sidek |
| title_sort | fabrication and characterization of flame retardant 4 and polydimethylsiloxane as substrates for a label-free dna sensor / irni hamiza hamzah, emilia noorsal, mohammad nizam ibrahim, asrulnizam abd manaf and othman sidek |
| topic | TK Electrical engineering. Electronics. Nuclear engineering |
| url | https://ir.uitm.edu.my/id/eprint/8898/ |