Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]
Metformin has anticancer effects by inhibiting glucose metabolism. Hyperthermia sensitizes cancer cells. It is known that both metformin,hypo- and hyperthermia deregulate mitochondrial metabolism in cancer. However, the mechanism of the additive effect is not known. The combined effect of metformin...
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| Format: | Research Reports |
| Language: | English |
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Research Management Institute (RMI)
2013
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/26751/ |
| _version_ | 1848806595098574848 |
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| author | Mahmood Al- Khateeb, Alyaa R Froemming, Gabriele Ruth Anisah Mohd Ismail, Aletza Abdul Hamid Hasani, Narimah |
| author_facet | Mahmood Al- Khateeb, Alyaa R Froemming, Gabriele Ruth Anisah Mohd Ismail, Aletza Abdul Hamid Hasani, Narimah |
| author_sort | Mahmood Al- Khateeb, Alyaa R |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Metformin has anticancer effects by inhibiting glucose metabolism. Hyperthermia sensitizes cancer cells. It is known that both metformin,hypo- and hyperthermia deregulate mitochondrial metabolism in cancer. However, the mechanism of the additive effect is not known. The combined effect of metformin and hypo- or hyperthermia on osteosarcoma cell line (MG-63 cell) viability, DNA damage, glucose metabolism, regulation of apoptotic genes and proteins were investigated. Osteosarcoma (MG-63) cells were treated with metformin IC50 30M for 48h followed by exposure to 45°C, 39°C, 35°C 27°C for 30 min, 1h and 2h , 37°C was served as control. The combinations of metformin with hypo- and hyperthermia significantly downregulated the expression of AKT1 and GSK3(3 genes, which led to a reduce cells viability and an increase in DNA damage. Metformin induced apoptosis and necrosis. The combined effect caused a downregulation of anti-apoptotic Bcl-2. The intrinsic pathway was not activated. Therefore, this study suggests that the combination of metformin with hyperthermia particularly 45°C, enhances apoptosis via Bax/Bid-dependent pathway. |
| first_indexed | 2025-11-14T22:29:25Z |
| format | Research Reports |
| id | uitm-26751 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T22:29:25Z |
| publishDate | 2013 |
| publisher | Research Management Institute (RMI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-267512023-07-13T07:17:33Z https://ir.uitm.edu.my/id/eprint/26751/ Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.] Mahmood Al- Khateeb, Alyaa R Froemming, Gabriele Ruth Anisah Mohd Ismail, Aletza Abdul Hamid Hasani, Narimah RC0254 Neoplasms. Tumors. Oncology (including Cancer) Metformin has anticancer effects by inhibiting glucose metabolism. Hyperthermia sensitizes cancer cells. It is known that both metformin,hypo- and hyperthermia deregulate mitochondrial metabolism in cancer. However, the mechanism of the additive effect is not known. The combined effect of metformin and hypo- or hyperthermia on osteosarcoma cell line (MG-63 cell) viability, DNA damage, glucose metabolism, regulation of apoptotic genes and proteins were investigated. Osteosarcoma (MG-63) cells were treated with metformin IC50 30M for 48h followed by exposure to 45°C, 39°C, 35°C 27°C for 30 min, 1h and 2h , 37°C was served as control. The combinations of metformin with hypo- and hyperthermia significantly downregulated the expression of AKT1 and GSK3(3 genes, which led to a reduce cells viability and an increase in DNA damage. Metformin induced apoptosis and necrosis. The combined effect caused a downregulation of anti-apoptotic Bcl-2. The intrinsic pathway was not activated. Therefore, this study suggests that the combination of metformin with hyperthermia particularly 45°C, enhances apoptosis via Bax/Bid-dependent pathway. Research Management Institute (RMI) 2013 Research Reports NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/26751/1/LP_ALYAA%20R%20MAHMOOD%20AL-%20KHATEEB%20RMI%2013_5.pdf Mahmood Al- Khateeb, Alyaa R and Froemming, Gabriele Ruth Anisah and Mohd Ismail, Aletza and Abdul Hamid Hasani, Narimah (2013) Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]. (2013) [Research Reports] (Unpublished) |
| spellingShingle | RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mahmood Al- Khateeb, Alyaa R Froemming, Gabriele Ruth Anisah Mohd Ismail, Aletza Abdul Hamid Hasani, Narimah Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.] |
| title | Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.] |
| title_full | Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.] |
| title_fullStr | Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.] |
| title_full_unstemmed | Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.] |
| title_short | Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.] |
| title_sort | targeting heat shock response in osteosarcoma cells by combination treatment / dr. alyaa r mahmood al- khateeb … [et al.] |
| topic | RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | https://ir.uitm.edu.my/id/eprint/26751/ |