Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl.

The inhibition of carbohydrate‐hydrolyzing enzymes in human digestive organs is crucial in controlling blood sugar levels, which is important in treating type 2 diabetes. In the current study, pahangensin A (1), a bis‐labdanic diterpene characterized previously in the rhizomes of Alpinia pahangensis...

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Main Authors: Loo, Kong Yong, Leong, Kok Hoong, Yasodha Sivasothy, *, Halijah Ibrahim, Khalijah Awang
Format: Article
Language:English
Published: Wiley 2019
Subjects:
Online Access:http://eprints.sunway.edu.my/1121/
http://eprints.sunway.edu.my/1121/1/Yasodha%20Chemistry%20%20Biodiversity-accepted%202019.pdf
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author Loo, Kong Yong
Leong, Kok Hoong
Yasodha Sivasothy, *
Halijah Ibrahim,
Khalijah Awang,
author_facet Loo, Kong Yong
Leong, Kok Hoong
Yasodha Sivasothy, *
Halijah Ibrahim,
Khalijah Awang,
author_sort Loo, Kong Yong
building SU Institutional Repository
collection Online Access
description The inhibition of carbohydrate‐hydrolyzing enzymes in human digestive organs is crucial in controlling blood sugar levels, which is important in treating type 2 diabetes. In the current study, pahangensin A (1), a bis‐labdanic diterpene characterized previously in the rhizomes of Alpinia pahangensis Ridl., was identified as an active dual inhibitor for α‐amylase (IC50=114.80 μm) and α‐glucosidase (IC50=153.87 μm). This is the first report on the dual α‐amylase and α‐glucosidase inhibitory activities of a bis‐labdanic diterpene. The Lineweaver‐Burk plots of compound 1 indicate that it is a mixed‐type inhibitor with regard to both enzymes. Based on molecular docking studies, compound 1 docked in a non‐active site of both enzymes. The dual inhibitory activity of compound 1 makes it a suitable natural alternative in the treatment of type 2 diabetes.
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publishDate 2019
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spelling sunway-11212019-11-15T01:45:48Z http://eprints.sunway.edu.my/1121/ Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl. Loo, Kong Yong Leong, Kok Hoong Yasodha Sivasothy, * Halijah Ibrahim, Khalijah Awang, QD Chemistry The inhibition of carbohydrate‐hydrolyzing enzymes in human digestive organs is crucial in controlling blood sugar levels, which is important in treating type 2 diabetes. In the current study, pahangensin A (1), a bis‐labdanic diterpene characterized previously in the rhizomes of Alpinia pahangensis Ridl., was identified as an active dual inhibitor for α‐amylase (IC50=114.80 μm) and α‐glucosidase (IC50=153.87 μm). This is the first report on the dual α‐amylase and α‐glucosidase inhibitory activities of a bis‐labdanic diterpene. The Lineweaver‐Burk plots of compound 1 indicate that it is a mixed‐type inhibitor with regard to both enzymes. Based on molecular docking studies, compound 1 docked in a non‐active site of both enzymes. The dual inhibitory activity of compound 1 makes it a suitable natural alternative in the treatment of type 2 diabetes. Wiley 2019-06 Article PeerReviewed text en cc_by_nc_nd_4 http://eprints.sunway.edu.my/1121/1/Yasodha%20Chemistry%20%20Biodiversity-accepted%202019.pdf Loo, Kong Yong and Leong, Kok Hoong and Yasodha Sivasothy, * and Halijah Ibrahim, and Khalijah Awang, (2019) Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl. Chemistry & Biodiversity, 16 (6). e1900032. ISSN 1612-1872 http://doi.org/10.1002/cbdv.201900032 doi:10.1002/cbdv.201900032
spellingShingle QD Chemistry
Loo, Kong Yong
Leong, Kok Hoong
Yasodha Sivasothy, *
Halijah Ibrahim,
Khalijah Awang,
Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl.
title Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl.
title_full Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl.
title_fullStr Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl.
title_full_unstemmed Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl.
title_short Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl.
title_sort molecular insight and mode of inhibition of α ‐glucosidase and α ‐amylase by pahangensin a from alpinia pahangensis ridl.
topic QD Chemistry
url http://eprints.sunway.edu.my/1121/
http://eprints.sunway.edu.my/1121/
http://eprints.sunway.edu.my/1121/
http://eprints.sunway.edu.my/1121/1/Yasodha%20Chemistry%20%20Biodiversity-accepted%202019.pdf