Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.)
Yeasts are potential biocontrol agents for Aspergillus flavus, an aflatoxin-producing fungus that is present in various agricultural products, including nutmeg. This study aimed to obtain yeast isolates from nutmeg (seeds, pulps, and leaves), characterise them, and identify their antagonistic effect...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25062/ http://journalarticle.ukm.my/25062/1/SSB%2012.pdf |
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| author | Susilowati, Dwi N Deswanti, Cahyani Hasna Radiastuti, Nani Sri Rahajoeningsih, Suryadi, Yadi Supriadi, Nurul Hidayah, Sri Widawati, Setyowati, Ninik |
| author_facet | Susilowati, Dwi N Deswanti, Cahyani Hasna Radiastuti, Nani Sri Rahajoeningsih, Suryadi, Yadi Supriadi, Nurul Hidayah, Sri Widawati, Setyowati, Ninik |
| author_sort | Susilowati, Dwi N |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Yeasts are potential biocontrol agents for Aspergillus flavus, an aflatoxin-producing fungus that is present in various agricultural products, including nutmeg. This study aimed to obtain yeast isolates from nutmeg (seeds, pulps, and leaves), characterise them, and identify their antagonistic effects on A. flavus. The antagonistic activities toward A. flavus were determined by a dual-culture method. Moreover, the possible mechanism responsible for these antagonistic effects was also analysed. The results showed that 51 yeast isolates were successfully isolated from nutmeg. The inhibition percentages of 47.25 ± 1.66% (isolate DP 1341a) and 55.98 ± 1.31% (isolate DP 1342) were statistically significant (p < 0.05). The antagonistic mechanisms of the DP 1341a isolate were associated with the production of volatile organic compounds (32.79 ± 1.01%), a chitinolytic index (2.51 ± 0.55), and hyperparasitism but not toxin activity. Moreover, the DP 1342 isolate produced volatile organic compounds (54.33 ± 3.13%), exhibited toxin activity (2.74 ± 0.22) and exhibited hyperparasitism but did not exhibit chitinase activity. Molecular identification showed that the two yeast isolates (DP 1341a and DP 1342) were identified as Pseudozyma hubeiensis with sequence similarity > 99%. Therefore, the selected yeast isolates, P. hubeiensis DP 1341a and DP 1342, could be further developed as biological control agents for A. flavus. This finding will also be useful for improving biological control agents as an eco-friendly and economically viable disease management strategy. |
| first_indexed | 2025-11-15T01:03:01Z |
| format | Article |
| id | oai:generic.eprints.org:25062 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:03:01Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:250622025-04-14T05:29:16Z http://journalarticle.ukm.my/25062/ Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.) Susilowati, Dwi N Deswanti, Cahyani Hasna Radiastuti, Nani Sri Rahajoeningsih, Suryadi, Yadi Supriadi, Nurul Hidayah, Sri Widawati, Setyowati, Ninik Yeasts are potential biocontrol agents for Aspergillus flavus, an aflatoxin-producing fungus that is present in various agricultural products, including nutmeg. This study aimed to obtain yeast isolates from nutmeg (seeds, pulps, and leaves), characterise them, and identify their antagonistic effects on A. flavus. The antagonistic activities toward A. flavus were determined by a dual-culture method. Moreover, the possible mechanism responsible for these antagonistic effects was also analysed. The results showed that 51 yeast isolates were successfully isolated from nutmeg. The inhibition percentages of 47.25 ± 1.66% (isolate DP 1341a) and 55.98 ± 1.31% (isolate DP 1342) were statistically significant (p < 0.05). The antagonistic mechanisms of the DP 1341a isolate were associated with the production of volatile organic compounds (32.79 ± 1.01%), a chitinolytic index (2.51 ± 0.55), and hyperparasitism but not toxin activity. Moreover, the DP 1342 isolate produced volatile organic compounds (54.33 ± 3.13%), exhibited toxin activity (2.74 ± 0.22) and exhibited hyperparasitism but did not exhibit chitinase activity. Molecular identification showed that the two yeast isolates (DP 1341a and DP 1342) were identified as Pseudozyma hubeiensis with sequence similarity > 99%. Therefore, the selected yeast isolates, P. hubeiensis DP 1341a and DP 1342, could be further developed as biological control agents for A. flavus. This finding will also be useful for improving biological control agents as an eco-friendly and economically viable disease management strategy. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25062/1/SSB%2012.pdf Susilowati, Dwi N and Deswanti, Cahyani Hasna and Radiastuti, Nani and Sri Rahajoeningsih, and Suryadi, Yadi and Supriadi, and Nurul Hidayah, and Sri Widawati, and Setyowati, Ninik (2025) Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.). Sains Malaysiana, 54 (1). pp. 151-164. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num1_2025/contentsVol54num1_2025.html |
| spellingShingle | Susilowati, Dwi N Deswanti, Cahyani Hasna Radiastuti, Nani Sri Rahajoeningsih, Suryadi, Yadi Supriadi, Nurul Hidayah, Sri Widawati, Setyowati, Ninik Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.) |
| title | Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.) |
| title_full | Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.) |
| title_fullStr | Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.) |
| title_full_unstemmed | Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.) |
| title_short | Biological control of Aspergillus flavus with Pseudozyma hubeiensis yeast from nutmeg (Myristica fragrans Houtt.) |
| title_sort | biological control of aspergillus flavus with pseudozyma hubeiensis yeast from nutmeg (myristica fragrans houtt.) |
| url | http://journalarticle.ukm.my/25062/ http://journalarticle.ukm.my/25062/ http://journalarticle.ukm.my/25062/1/SSB%2012.pdf |