Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products
Microbiologically Induced Calcium Carbonate Precipitation (MICCP) through urea hydrolysis is the most effective way to precipitate a high concentration of calcium carbonate (CaCO3) within a short time. The MICCP process is used to remediate the micro-crack in the concrete. However, limited research...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://journalarticle.ukm.my/22793/ http://journalarticle.ukm.my/22793/1/SEN%209.pdf |
| _version_ | 1848815686171754496 |
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| author | Nurul Asyiqin Addenan, Mohamad Syazwan Ngalimat, Raja Noor Zaliha Raja Abd Rahman, Rakesh Donepudi, Noor Azline Mohd Nasir, Mohd Saleh Jaafar, Suriana Sabri, |
| author_facet | Nurul Asyiqin Addenan, Mohamad Syazwan Ngalimat, Raja Noor Zaliha Raja Abd Rahman, Rakesh Donepudi, Noor Azline Mohd Nasir, Mohd Saleh Jaafar, Suriana Sabri, |
| author_sort | Nurul Asyiqin Addenan, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Microbiologically Induced Calcium Carbonate Precipitation (MICCP) through urea hydrolysis is the most effective way to precipitate a high concentration of calcium carbonate (CaCO3) within a short time. The MICCP process is used to remediate the micro-crack in the concrete. However, limited research has been conducted to determine CaCO3 precipitation by bacteria, especially in Malaysia. Here, Bacillus spp. isolated from the Malaysian stingless bee products were evaluated for CaCO3 precipitation. Bacillus spp. were selected for further study according to their ability to produce urease enzymes. The urease-positive Bacillus spp. were screened for CaCO3 precipitation by culturing on both CaCO3 precipitation agar and broth media. The survivability of the urease-positive Bacillus spp. in various temperatures, pH values, and NaCl concentrations were tested. Seven out of 11 Bacillus spp. were found as ureolytic bacteria. Among the ureolytic bacteria, bacteria belonging to the Bacillus subtilis species complex group showed the highest number of bacteria (36.4%) that are capable of precipitating CaCO3. Bacillus stratosphericus PD6 and B. aryabhattai BD8 exhibited the largest CaCO3 precipitation zones (15 mm). Bacillus stratosphericus PD6 also precipitated the highest amount of CaCO3 (65 mg) and urease activity (0.197 U/mL). All the urease-positive Bacillus spp. were able to grow at 45 °C, pH (8 to 12), and 5% NaCl. Only B. subtilis BD3 can withstand high temperatures up to 55 °C and 15% NaCl concentration. In conclusion, Bacillus spp. isolated from stingless bee products showed the ability as the CaCO3 precipitating bacteria; suggesting its potential application in self-healing concrete. |
| first_indexed | 2025-11-15T00:53:55Z |
| format | Article |
| id | oai:generic.eprints.org:22793 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:53:55Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:227932024-01-03T03:28:16Z http://journalarticle.ukm.my/22793/ Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products Nurul Asyiqin Addenan, Mohamad Syazwan Ngalimat, Raja Noor Zaliha Raja Abd Rahman, Rakesh Donepudi, Noor Azline Mohd Nasir, Mohd Saleh Jaafar, Suriana Sabri, Microbiologically Induced Calcium Carbonate Precipitation (MICCP) through urea hydrolysis is the most effective way to precipitate a high concentration of calcium carbonate (CaCO3) within a short time. The MICCP process is used to remediate the micro-crack in the concrete. However, limited research has been conducted to determine CaCO3 precipitation by bacteria, especially in Malaysia. Here, Bacillus spp. isolated from the Malaysian stingless bee products were evaluated for CaCO3 precipitation. Bacillus spp. were selected for further study according to their ability to produce urease enzymes. The urease-positive Bacillus spp. were screened for CaCO3 precipitation by culturing on both CaCO3 precipitation agar and broth media. The survivability of the urease-positive Bacillus spp. in various temperatures, pH values, and NaCl concentrations were tested. Seven out of 11 Bacillus spp. were found as ureolytic bacteria. Among the ureolytic bacteria, bacteria belonging to the Bacillus subtilis species complex group showed the highest number of bacteria (36.4%) that are capable of precipitating CaCO3. Bacillus stratosphericus PD6 and B. aryabhattai BD8 exhibited the largest CaCO3 precipitation zones (15 mm). Bacillus stratosphericus PD6 also precipitated the highest amount of CaCO3 (65 mg) and urease activity (0.197 U/mL). All the urease-positive Bacillus spp. were able to grow at 45 °C, pH (8 to 12), and 5% NaCl. Only B. subtilis BD3 can withstand high temperatures up to 55 °C and 15% NaCl concentration. In conclusion, Bacillus spp. isolated from stingless bee products showed the ability as the CaCO3 precipitating bacteria; suggesting its potential application in self-healing concrete. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22793/1/SEN%209.pdf Nurul Asyiqin Addenan, and Mohamad Syazwan Ngalimat, and Raja Noor Zaliha Raja Abd Rahman, and Rakesh Donepudi, and Noor Azline Mohd Nasir, and Mohd Saleh Jaafar, and Suriana Sabri, (2023) Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products. Sains Malaysiana, 52 (6). pp. 1723-1735. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num6_2023/contentsVol52num6_2023.html |
| spellingShingle | Nurul Asyiqin Addenan, Mohamad Syazwan Ngalimat, Raja Noor Zaliha Raja Abd Rahman, Rakesh Donepudi, Noor Azline Mohd Nasir, Mohd Saleh Jaafar, Suriana Sabri, Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products |
| title | Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products |
| title_full | Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products |
| title_fullStr | Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products |
| title_full_unstemmed | Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products |
| title_short | Evaluation of calcium carbonate precipitation by Bacillus spp. isolated from stingless bee products |
| title_sort | evaluation of calcium carbonate precipitation by bacillus spp. isolated from stingless bee products |
| url | http://journalarticle.ukm.my/22793/ http://journalarticle.ukm.my/22793/ http://journalarticle.ukm.my/22793/1/SEN%209.pdf |