Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia
White Spot Disease (WSD) is a viral disease affecting crustaceans. Caused by the White Spot Syndrome Virus (WSSV), this disease has caused significant mortality in commercially cultivated marine shrimp species with severe impacts on the shrimp farming industry and may be a threat to wild shrimp...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20636/ http://journalarticle.ukm.my/20636/1/4.pdf |
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| author | Tasakka, A.C.M.A.R. Latama, G. Hidayani, A.A. Parenrengi, A. Tenriulo, A. Relatami, A.N.R. Moore, A.M. Shaheen, A. |
| author_facet | Tasakka, A.C.M.A.R. Latama, G. Hidayani, A.A. Parenrengi, A. Tenriulo, A. Relatami, A.N.R. Moore, A.M. Shaheen, A. |
| author_sort | Tasakka, A.C.M.A.R. |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | White Spot Disease (WSD) is a viral disease affecting crustaceans. Caused by the White Spot Syndrome Virus (WSSV),
this disease has caused significant mortality in commercially cultivated marine shrimp species with severe impacts on
the shrimp farming industry and may be a threat to wild shrimp stocks. Thorough studies on the molecular biology of
this pathogen are urgently needed to improve understanding of the virus at a molecular level, including variation in
key viral protein (VP) components of the WSSV virion. This study aimed to isolate and characterize WSSV VP28 gene
encoding envelope proteins from Indonesian Pacific white shrimp (Litopenaeus vannamei) isolates. Infected juvenile
shrimp were collected from Pangkep, Barru, and Pinrang Districts in South Sulawesi, Indonesia. Genomic DNA was
isolated from infected shrimp muscle tissue using a DTAB-CTAB (dodecyle trimethyl ammonium bromide-hexadecyl
trimethyl ammonium bromide) DNA extraction procedure. The WSSV VP28 DNA sequences from Pangkep, Barru,
and Pinrang isolates were 640-680 bp in length. Homology of Pangkep isolates with isolates from Barru and Pinrang
was 97-99%. BLAST-N (Basic Local Alignment Search Tool-Nucleotide) analysis showed isolates from all three sites
clustered with WSSV VP28 accessions from China, Indonesia, Japan, South Carolina and Vietnam. These results increase
the geographic spread and host taxon coverage of WSSV VP28 sequence data for Indonesia. |
| first_indexed | 2025-11-15T00:45:28Z |
| format | Article |
| id | oai:generic.eprints.org:20636 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:45:28Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:206362022-12-06T00:51:16Z http://journalarticle.ukm.my/20636/ Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia Tasakka, A.C.M.A.R. Latama, G. Hidayani, A.A. Parenrengi, A. Tenriulo, A. Relatami, A.N.R. Moore, A.M. Shaheen, A. White Spot Disease (WSD) is a viral disease affecting crustaceans. Caused by the White Spot Syndrome Virus (WSSV), this disease has caused significant mortality in commercially cultivated marine shrimp species with severe impacts on the shrimp farming industry and may be a threat to wild shrimp stocks. Thorough studies on the molecular biology of this pathogen are urgently needed to improve understanding of the virus at a molecular level, including variation in key viral protein (VP) components of the WSSV virion. This study aimed to isolate and characterize WSSV VP28 gene encoding envelope proteins from Indonesian Pacific white shrimp (Litopenaeus vannamei) isolates. Infected juvenile shrimp were collected from Pangkep, Barru, and Pinrang Districts in South Sulawesi, Indonesia. Genomic DNA was isolated from infected shrimp muscle tissue using a DTAB-CTAB (dodecyle trimethyl ammonium bromide-hexadecyl trimethyl ammonium bromide) DNA extraction procedure. The WSSV VP28 DNA sequences from Pangkep, Barru, and Pinrang isolates were 640-680 bp in length. Homology of Pangkep isolates with isolates from Barru and Pinrang was 97-99%. BLAST-N (Basic Local Alignment Search Tool-Nucleotide) analysis showed isolates from all three sites clustered with WSSV VP28 accessions from China, Indonesia, Japan, South Carolina and Vietnam. These results increase the geographic spread and host taxon coverage of WSSV VP28 sequence data for Indonesia. Penerbit Universiti Kebangsaan Malaysia 2022-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20636/1/4.pdf Tasakka, A.C.M.A.R. and Latama, G. and Hidayani, A.A. and Parenrengi, A. and Tenriulo, A. and Relatami, A.N.R. and Moore, A.M. and Shaheen, A. (2022) Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia. Sains Malaysiana, 51 (9). pp. 2775-2788. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil9_2022/KandunganJilid51Bil9_2022.html |
| spellingShingle | Tasakka, A.C.M.A.R. Latama, G. Hidayani, A.A. Parenrengi, A. Tenriulo, A. Relatami, A.N.R. Moore, A.M. Shaheen, A. Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia |
| title | Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia |
| title_full | Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia |
| title_fullStr | Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia |
| title_full_unstemmed | Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia |
| title_short | Variability of White Spot Syndrome Virus (WSSV) envelope protein VP28 from diseased shrimp (Litopenaeus vannamei) in Indonesia |
| title_sort | variability of white spot syndrome virus (wssv) envelope protein vp28 from diseased shrimp (litopenaeus vannamei) in indonesia |
| url | http://journalarticle.ukm.my/20636/ http://journalarticle.ukm.my/20636/ http://journalarticle.ukm.my/20636/1/4.pdf |