Biosynthesis of gold nanoparticles (AuNPs) by Diopatra claparedii grube, 1878 (Polychaeta: Onuphidae) and its antibacterial activity
Gold nanoparticles (AuNPs) have gained attention as it possesses outstanding physicochemical properties, and utilised in variety of applications especially in biomedical and pharmaceutical. Majorly, AuNPs are produced by conventional methods (chemical and physical). However, these methods bring...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17408/ http://journalarticle.ukm.my/17408/1/11.pdf |
| Summary: | Gold nanoparticles (AuNPs) have gained attention as it possesses outstanding physicochemical properties, and utilised
in variety of applications especially in biomedical and pharmaceutical. Majorly, AuNPs are produced by conventional
methods (chemical and physical). However, these methods bring several drawbacks such as toxic, hazardous, low
yield and non-environmental friendly. Hence, biosynthesis of AuNPs that compliance with ‘greener’ approach becomes
vitals. In this study, marine tube worm of Diopatra claparedii (polychaetes) was employed as reducing agent in the
biosynthesis of AuNPs. The biosynthesised of AuNPs by D. claparedii extract was successfully prepared under
ambient temperature and normal atmospheric conditions. The formation of AuNPs was confirmed by the appearance of
surface Plasmon resonance (SPR) bands around 540 to 560 nm characterized by UV-Vis spectroscopy. Scanning electron
microscopy (SEM) showed that the AuNPs are mostly in agglomerated spherical like shapes with size ranging from
100 to 400 nm. Meanwhile, transmission electron microscopy (TEM) showed that the particles are in the size range from
25 to 60 nm, also mainly form in spherical like shape. The particle size of AuNPs in a range of 50-100 nm was showed
by dynamic light scattering (DLS). Fourier-transform infrared (FTIR) spectrum of D. claparedii extract indicated the
existence of several functional groups. Biosynthesised AuNPs also successfully gave inhibition on bacterial growth
(Staphyloccus aureus, S. epidermidis, Escheratia coli, Salmonella typhi) through antibacterial assessment. |
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