Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth
Cell suspension culture of Citrus suhuiensis (C. suhuiensis) was established to determine the best growth condition based on the effects of carbon source, plant growth regulators (PGRs) and inoculum size. Friable callus from the cotyledon explant was used to initiate the cell suspension cultures....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15703/ http://journalarticle.ukm.my/15703/1/49_01_10.pdf |
| _version_ | 1848813863909195776 |
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| author | Noor Illi Mohamad Puad, Nur Alia M. Fathil, Azura Amid, Azlin Suhaida Azmi, |
| author_facet | Noor Illi Mohamad Puad, Nur Alia M. Fathil, Azura Amid, Azlin Suhaida Azmi, |
| author_sort | Noor Illi Mohamad Puad, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Cell suspension culture of Citrus suhuiensis (C. suhuiensis) was established to determine the best growth condition based
on the effects of carbon source, plant growth regulators (PGRs) and inoculum size. Friable callus from the cotyledon explant
was used to initiate the cell suspension cultures. Murashige and Skoog (MS) medium supplemented with 50 g/L glucose
achieved the highest cell dry weight (CDW) and µmax of 23.2 g/L and 0.47 h-1 respectively compared to glucose at 30 g/L
and other carbon sources. For PGRs effects, 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) gave the highest amount of
CDW and µmax; 11.91 g/L and 0.32 h-1. Furthermore, the inoculum size at 10% (v/v) gave the maximum CDW and µmax of
15.45 g/L and 0.71 h-1 respectively compared to 20% (v/v) and 30% (v/v) of inoculum. The results achieved can be used for
further statistical optimization of C. suhuiensis cell suspension culture growth parameters in which the interactions between
those parameters can be further studied and understood. |
| first_indexed | 2025-11-15T00:24:57Z |
| format | Article |
| id | oai:generic.eprints.org:15703 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:24:57Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:157032020-11-18T05:54:57Z http://journalarticle.ukm.my/15703/ Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth Noor Illi Mohamad Puad, Nur Alia M. Fathil, Azura Amid, Azlin Suhaida Azmi, Cell suspension culture of Citrus suhuiensis (C. suhuiensis) was established to determine the best growth condition based on the effects of carbon source, plant growth regulators (PGRs) and inoculum size. Friable callus from the cotyledon explant was used to initiate the cell suspension cultures. Murashige and Skoog (MS) medium supplemented with 50 g/L glucose achieved the highest cell dry weight (CDW) and µmax of 23.2 g/L and 0.47 h-1 respectively compared to glucose at 30 g/L and other carbon sources. For PGRs effects, 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) gave the highest amount of CDW and µmax; 11.91 g/L and 0.32 h-1. Furthermore, the inoculum size at 10% (v/v) gave the maximum CDW and µmax of 15.45 g/L and 0.71 h-1 respectively compared to 20% (v/v) and 30% (v/v) of inoculum. The results achieved can be used for further statistical optimization of C. suhuiensis cell suspension culture growth parameters in which the interactions between those parameters can be further studied and understood. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15703/1/49_01_10.pdf Noor Illi Mohamad Puad, and Nur Alia M. Fathil, and Azura Amid, and Azlin Suhaida Azmi, (2020) Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth. Malaysian Applied Biology, 49 (1). pp. 87-93. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56 |
| spellingShingle | Noor Illi Mohamad Puad, Nur Alia M. Fathil, Azura Amid, Azlin Suhaida Azmi, Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_full | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_fullStr | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_full_unstemmed | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_short | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_sort | effects of carbon sources, plant growth regulators and inoculum size on citrus suhuiensis cell suspension culture growth |
| url | http://journalarticle.ukm.my/15703/ http://journalarticle.ukm.my/15703/ http://journalarticle.ukm.my/15703/1/49_01_10.pdf |