Microscopic observation of a root-adhering factor Avin_16040 in Azotobacter vinelandii and Escherichia coli during association with rice root
Microbial adherence to plant root is the initial step in a beneficial plant-microbe interaction. Quantitative RT-PCR analysis deduced the Avin_16040 gene showed upregulated expression when Azotobacter vinelandii was adhered to the rice root. By transforming the full-length Avin_16040 gene into a h...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12332/ http://journalarticle.ukm.my/12332/1/46_02_07.pdf |
| _version_ | 1848812972773736448 |
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| author | Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan, |
| author_facet | Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan, |
| author_sort | Liew, Pauline Woan Ying |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Microbial adherence to plant root is the initial step in a beneficial plant-microbe interaction. Quantitative RT-PCR analysis
deduced the Avin_16040 gene showed upregulated expression when Azotobacter vinelandii was adhered to the rice root. By
transforming the full-length Avin_16040 gene into a heterologous host Escherichia coli, the recombinant clones displayed
filamentous cell shapes in contrast to the rod-shape of wild type cells. Besides full-length gene insert, some E. coli clones
were detected to contain truncated Avin_16040 gene inserts but still shape-shifted to filamentous cells. Further analysis by
DNA sequencing revealed the shape-shifting E. coli clones contained 3’-end truncated Avin_16040 gene, while E. coli clones
containing the 5’-end truncated Avin_16040 gene remained rod-shaped. The cell surface topographies of A. vinelandii and E.
coli cells in the presence and absence of Avin_16040 gene and in association with rice root adherence were analysed using
atomic force microscopy. |
| first_indexed | 2025-11-15T00:10:47Z |
| format | Article |
| id | oai:generic.eprints.org:12332 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:10:47Z |
| publishDate | 2017 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:123322018-11-16T21:19:16Z http://journalarticle.ukm.my/12332/ Microscopic observation of a root-adhering factor Avin_16040 in Azotobacter vinelandii and Escherichia coli during association with rice root Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan, Microbial adherence to plant root is the initial step in a beneficial plant-microbe interaction. Quantitative RT-PCR analysis deduced the Avin_16040 gene showed upregulated expression when Azotobacter vinelandii was adhered to the rice root. By transforming the full-length Avin_16040 gene into a heterologous host Escherichia coli, the recombinant clones displayed filamentous cell shapes in contrast to the rod-shape of wild type cells. Besides full-length gene insert, some E. coli clones were detected to contain truncated Avin_16040 gene inserts but still shape-shifted to filamentous cells. Further analysis by DNA sequencing revealed the shape-shifting E. coli clones contained 3’-end truncated Avin_16040 gene, while E. coli clones containing the 5’-end truncated Avin_16040 gene remained rod-shaped. The cell surface topographies of A. vinelandii and E. coli cells in the presence and absence of Avin_16040 gene and in association with rice root adherence were analysed using atomic force microscopy. Penerbit Universiti Kebangsaan Malaysia 2017-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12332/1/46_02_07.pdf Liew, Pauline Woan Ying and Jong, Bor Chyan and Najimudin Nazalan, (2017) Microscopic observation of a root-adhering factor Avin_16040 in Azotobacter vinelandii and Escherichia coli during association with rice root. Malaysian Applied Biology, 46 (2). pp. 49-58. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=644&catid=59:current-view&Itemid=56 |
| spellingShingle | Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan, Microscopic observation of a root-adhering factor Avin_16040 in Azotobacter vinelandii and Escherichia coli during association with rice root |
| title | Microscopic observation of a root-adhering factor
Avin_16040 in Azotobacter vinelandii and Escherichia coli
during association with rice root |
| title_full | Microscopic observation of a root-adhering factor
Avin_16040 in Azotobacter vinelandii and Escherichia coli
during association with rice root |
| title_fullStr | Microscopic observation of a root-adhering factor
Avin_16040 in Azotobacter vinelandii and Escherichia coli
during association with rice root |
| title_full_unstemmed | Microscopic observation of a root-adhering factor
Avin_16040 in Azotobacter vinelandii and Escherichia coli
during association with rice root |
| title_short | Microscopic observation of a root-adhering factor
Avin_16040 in Azotobacter vinelandii and Escherichia coli
during association with rice root |
| title_sort | microscopic observation of a root-adhering factor
avin_16040 in azotobacter vinelandii and escherichia coli
during association with rice root |
| url | http://journalarticle.ukm.my/12332/ http://journalarticle.ukm.my/12332/ http://journalarticle.ukm.my/12332/1/46_02_07.pdf |