Cas3 nuclease activity is regulated by an iHDA1 region located at the interface between HD and RecA1 domain

QR Code

Cas3 nuclease activity is regulated by an iHDA1 region located at the interface between HD and RecA1 domain

CRISPR Cas3 is a crucial protein effector for type I CRISPR interference. It couples actions of helicase and nuclease domains to eliminate mobile genetic elements (MGEs) in response to the recognition of protospacer sequences on foreign DNA by the Cascade complex that recruits Cas3. The DNA cleavage...

Full description

Bibliographic Details
Main Author:	He, Liu
Format:	Thesis (University of Nottingham only)
Language:	English
Published:	2022
Subjects:	CRISPR; Genetics; Mobile genetic elements; Prokaryotes; Nucleases
Online Access:	https://eprints.nottingham.ac.uk/69106/

Similar Items

Characterising Host Factors Required for CRISPR Cas Adaptation in Escherichia coli
by: Edwards, Harry
Published: (2023)

The role of Cas8 in type I CRISPR interference
by: Cass, Simon, et al.
Published: (2015)

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3
by: Gong, Bei, et al.
Published: (2014)

Investigation of CRISPR-Cas adaptation mechanism through exploration of Cas4-1 fusions
by: Walker, Emily
Published: (2019)

CRISPR-Cas9-mediated genome editing of the extracellular matrix gene TNC in human fibroblasts
by: Brunetti, Giulio
Published: (2017)

RNA-guided integration of diverse DNA molecules by casposase-Cas9 fusions
by: Lau, Chun H.
Published: (2020)

Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells
by: Kondrashov, Alexander, et al.
Published: (2018)

Analysis of Cas1-2 and Cas3 CRISPR immunity proteins in E. coli cell physiology
by: Reeves, Ryan Peter
Published: (2019)

Association of glucokinase, glucokinase regulator, glucose-6-phosphatase catalytic subunit 2 and melatonin receptor 1b genes polymorphisms with type 2 diabetic subjects
by: Ansari, Neda
Published: (2020)

Distribution of CCL3L1 copy number variable gene among three major ethnic groups in Malaysia
by: Jamaluddin, Jalilah
Published: (2017)

CRISPR-Cas Adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonised by 5’ ssDNA exonucleases
by: Killelea, Tom, et al.
Published: (2018)

Cadmium concentration and genetic polymorphism of GSTM1, GSTP1 and GSTT1 in blood samples of respondents from Melaka, Malaysia
by: Md Zulkifli, Hamizah
Published: (2018)

Mutation of recA gene of Vibrio cholerae: Towards the development of attenuated vibrio cholerae vaccine strains
by: Kuan, Goh Lai
Published: (2004)

The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter
by: Chan, Pick Kuen, et al.
Published: (2004)

Genetic Analysis of RadB, a Paralogue of the Archaeal Rad51/RecA Homologue, RadA
by: Haldenby, Sam
Published: (2007)

Copy Number Variation of FCGR3B on Negrito-Mendriq / Mohd Helmy Yusof, Umi Shakina Haridan and Muhamad Rahimi Che Hassan
by: Yusof, Mohd Helmy, et al.
Published: (2019)

Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS
by: Majsec, Kristina, et al.
Published: (2016)

Performance of kenaf (Hibiscus cannabinus L.) genotypes and their variability based on DNA micrositellite makers
by: Foroughi, Majid
Published: (2012)

Descriptive study on the origin and evolution of beta- defensins copy number variable gene using multigenerational family pedigrees
by: Musa, Nurul Azmah
Published: (2019)

Part A: Application of fluorescent markers onto CRISPR-associated proteins/ Part B: Substitution of cysteine residues in CRISPR associated Protein Cas1
by: Parr, Lochlan
Published: (2024)

Role of Transforming Growth Factor-Beta1 (TGFẞ-1) and the cell signaling pathway in the differentiation of SHED into epithelial-like cells
by: Ahmad, Azlina
Published: (2017)

Genetic potential of selected sweet corn inbred lines and analysis of their combining ability assisted by microsatellite DNA markers
by: Kashiani, Pedram
Published: (2012)

Association of GSTM1 and GSTT1 genetic polymorphism with blood methylmercury level and birth outcome among fish-eating urban pregnant women
by: Abedinlah, Amirah
Published: (2020)

Investigating the effects of altered Rad51 expression on the DSB repair pathway choice and the conservation of RecA homologs in Saccharomyces cerevisiae
by: Rodrigo, Mahari
Published: (2023)

Review on genetic transformation of rice / Nur Amalina Ghazali
by: Ghazali, Nur Amalina
Published: (2019)

Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults
by: Wan Omar, Wan Fatein Nabeila, et al.
Published: (2019)

Biomphalaria snails in East Africa’s river systems: distribution, genetics and schistosome infections
by: Onyango, Victor Magero
Published: (2024)

From shed skins to genetic insights: conservation genetics of the Fen Raft Spider (Dolomedes plantarius)
by: Maka, Anna
Published: (2024)

Isolation, Characterization and Genetic Linkage Mapping of Microsatellite Markers in Mystus Nemurus
by: Hoh, Boon Peng
Published: (2005)

Genetic diversity and heritability estimate of Jatropha curcas L. accessions using agro-morphological and molecular markers
by: Arolu, Ibrahim Wasiu
Published: (2012)

Genotype by environment interaction and genotype stability analyses on advanced fragrant rice lines derived from MR269 × basmati 370
by: Md Hashim, Norainy
Published: (2019)

Genetic identification of purebred and crossbred water buffalo [Bubalus bubalis (linnaeus, 1758)] in selected farms in Malaysia
by: Shaari, Nor 'Ammar Liyana
Published: (2019)

Effects of signal peptides on secretion of the bacillus g1 β-cyclodextrin glucanotransferase in lactococcus lactis NZ9000
by: Subramaniam, Menaga
Published: (2013)

Identification of candidate genes expressions and polymorphisms associated with atherosclerosis from postmortem cases in Hospital Kuala Lumpur and Hospital Serdang, Malaysia
by: Blin, Joan
Published: (2013)

Development of gene manipulation systems for green microalga (Ankistrodesmus convolutus corda.)
by: Thanh, Tran
Published: (2012)

Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363
by: Ahmat, Nurfilza
Published: (2001)

Nucleotide polymorphism of enhanced disease resistance 1 (edr1) from kelampayan (neolamarckia cadamba)
by: Tan, Soon Yu
Published: (2013)

Development of molecular tools for optimisation of C1 gas fermentation in acetogens
by: Rowe, Peter
Published: (2018)

Screening of Malaysian rice accessions and marker-assisted selection of a cross between MR263 × swarna-sub1 varieties for submergence tolerance
by: Mojulat, Welland Cosmas
Published: (2015)

Analysis of the E. coli CRISPR Cas3 protein and its potential for regulation by a short non-coding RNA
by: Kennerdale, Niamh
Published: (2024)