Cas3 nuclease activity is regulated by an iHDA1 region located at the interface between HD and RecA1 domain
CRISPR Cas3 is a crucial protein effector for type I CRISPR interference. It couples actions of helicase and nuclease domains to eliminate mobile genetic elements (MGEs) in response to the recognition of protospacer sequences on foreign DNA by the Cascade complex that recruits Cas3. The DNA cleavage...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2022
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| Online Access: | https://eprints.nottingham.ac.uk/69106/ |