Genetic screens in intestinal organoid (mini-gut) culture using CRISPR-Cas-9 System and studying the role of FBXL5 in colorectal cancer cells

Current in vitro research methods are often difficult to translate into successful gene(s) functional and clinical applications. Immortalised cell lines are monocellular, 2-dimensional and do not accurately encapsulate the in vivo microenvironment, while in vivo animal models are often impractical,...

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Bibliographic Details
Main Author: Jinks, Nicholas
Format: Thesis (University of Nottingham only)
Language:English
Published: 2022
Subjects:
Online Access:https://eprints.nottingham.ac.uk/68697/