Studies on the biochemistry of the Targeting domain of Lysostaphin

Lysostaphin from S. simulans was cloned in expression vector pET21a and expressed and purified in E .coli. A spot test and a broth dilution assay indicated that the minimum inhibitory concentration of lysostaphin required to kill EMRSA-16 was 40 nM. Lysostaphin consists of an endopeptidase and a tra...

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Bibliographic Details
Main Author: Antoniadou, Eleni
Format: Thesis (University of Nottingham only)
Language:English
Published: 2004
Subjects:
Online Access:https://eprints.nottingham.ac.uk/67471/