Development of a high-throughput screen for the study of CRISPR spacer acquisition

Bacteria are in an ever-evolving arms race to survive against invasion from foreign genetic elements. One mechanism of defence is the CRISPR-Cas system of adaptive immunity. Through CRISPR-Cas, cells store memories of previous invaders as DNA fragments, or spacers, in a genomic array of repeats. Onc...

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Bibliographic Details
Main Author: Braithwaite, Jack
Format: Thesis (University of Nottingham only)
Language:English
Published: 2021
Subjects:
Online Access:https://eprints.nottingham.ac.uk/66912/