CRISPR-Cas Adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonised by 5’ ssDNA exonucleases

CRISPR-Cas Adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonised by 5’ ssDNA exonucleases

Prokaryotic adaptive immunity is established against mobile genetic elements (MGEs) by “naïve adaptation” when DNA fragments from a newly encountered MGE are integrated into CRISPR-Cas systems. In E. coli, DNA integration catalysed by Cas1-Cas2 integrase is well understood in mechanistic and stru...

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Bibliographic Details
Main Authors: Killelea, Tom, Radovcic, Marin, Savitskaya, Ekaterina, Ivančić-Baće, Ivana, Bolt, Edward L.
Format: Article
Language:English
Published: Oxford Journals 2018
Online Access:https://eprints.nottingham.ac.uk/53448/

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