CRISPR-Cas Adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonised by 5’ ssDNA exonucleases
Prokaryotic adaptive immunity is established against mobile genetic elements (MGEs) by “naïve adaptation” when DNA fragments from a newly encountered MGE are integrated into CRISPR-Cas systems. In E. coli, DNA integration catalysed by Cas1-Cas2 integrase is well understood in mechanistic and stru...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Oxford Journals
2018
|
| Online Access: | https://eprints.nottingham.ac.uk/53448/ |