Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support
The use of an in-situ immobilization procedure for the immobilization of hyperhalophilic alcohol dehydrogenase in a metal organic framework material is described. The easy and rapid in situ immobilization process enables retention of activity over a broad range of pH and temperature together with a...
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| Format: | Article |
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ACS Publications
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52945/ |
| _version_ | 1848798844390735872 |
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| author | Carucci, Cristina Bruen, Larah Gascón, Victoria Paradisi, Francesca Magner, Edmond |
| author_facet | Carucci, Cristina Bruen, Larah Gascón, Victoria Paradisi, Francesca Magner, Edmond |
| author_sort | Carucci, Cristina |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The use of an in-situ immobilization procedure for the immobilization of hyperhalophilic alcohol dehydrogenase in a metal organic framework material is described. The easy and rapid in situ immobilization process enables retention of activity over a broad range of pH and temperature together with a decrease in the halophilicity of the enzyme. The catalytic activity of the immobilized enzyme was studied in non-aqueous solvent mixtures with the highest retention of activity in aqueous solutions of methanol and acetonitrile. The approach demonstrates that this immobilization method can be extended to hyperhalophilic enzymes with enhancements in activity and stability. |
| first_indexed | 2025-11-14T20:26:14Z |
| format | Article |
| id | nottingham-52945 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:26:14Z |
| publishDate | 2018 |
| publisher | ACS Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-529452020-05-04T19:41:17Z https://eprints.nottingham.ac.uk/52945/ Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support Carucci, Cristina Bruen, Larah Gascón, Victoria Paradisi, Francesca Magner, Edmond The use of an in-situ immobilization procedure for the immobilization of hyperhalophilic alcohol dehydrogenase in a metal organic framework material is described. The easy and rapid in situ immobilization process enables retention of activity over a broad range of pH and temperature together with a decrease in the halophilicity of the enzyme. The catalytic activity of the immobilized enzyme was studied in non-aqueous solvent mixtures with the highest retention of activity in aqueous solutions of methanol and acetonitrile. The approach demonstrates that this immobilization method can be extended to hyperhalophilic enzymes with enhancements in activity and stability. ACS Publications 2018-06-19 Article PeerReviewed Carucci, Cristina, Bruen, Larah, Gascón, Victoria, Paradisi, Francesca and Magner, Edmond (2018) Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support. Langmuir . ISSN 0743-7463 https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.8b01037 doi:10.1021/acs.langmuir.8b01037 doi:10.1021/acs.langmuir.8b01037 |
| spellingShingle | Carucci, Cristina Bruen, Larah Gascón, Victoria Paradisi, Francesca Magner, Edmond Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support |
| title | Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support |
| title_full | Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support |
| title_fullStr | Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support |
| title_full_unstemmed | Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support |
| title_short | Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support |
| title_sort | significant enhancement of structural stability of the hyperhalophilic adh from haloferax volcanii via entrapment on metal organic framework support |
| url | https://eprints.nottingham.ac.uk/52945/ https://eprints.nottingham.ac.uk/52945/ https://eprints.nottingham.ac.uk/52945/ |