Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms

Atmospheric air plasma has previously been shown to be a novel and effective method for biofilm eradication. Here we study the effects of plasma on both microbial inactivation and induced structural modification for forming biofilms. New structures are created from aggregates of extracellular polysa...

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Main Authors: Kwandou, Goldina, Mai-Prochnow, Anne, Prescott, Stuart W., Spicer, Patrick T., Cullen, P.J.
Format: Article
Published: Wiley-VCH Verlag 2018
Subjects:
Online Access:https://eprints.nottingham.ac.uk/52768/
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author Kwandou, Goldina
Mai-Prochnow, Anne
Prescott, Stuart W.
Spicer, Patrick T.
Cullen, P.J.
author_facet Kwandou, Goldina
Mai-Prochnow, Anne
Prescott, Stuart W.
Spicer, Patrick T.
Cullen, P.J.
author_sort Kwandou, Goldina
building Nottingham Research Data Repository
collection Online Access
description Atmospheric air plasma has previously been shown to be a novel and effective method for biofilm eradication. Here we study the effects of plasma on both microbial inactivation and induced structural modification for forming biofilms. New structures are created from aggregates of extracellular polysaccharides and dead bacterial cells, forming a protective and resilient matrix in which the remaining living cells grow and reproduce under proper growth conditions. The new colonies are found to be more resilient in this state, reducing the efficacy of subsequent plasma treatment. We verify that the observed effect is not caused by chemicals produced by plasma reactive species, but instead by the physical processes of drying and convection caused by the plasma discharge.
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spelling nottingham-527682020-05-04T19:40:12Z https://eprints.nottingham.ac.uk/52768/ Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms Kwandou, Goldina Mai-Prochnow, Anne Prescott, Stuart W. Spicer, Patrick T. Cullen, P.J. Atmospheric air plasma has previously been shown to be a novel and effective method for biofilm eradication. Here we study the effects of plasma on both microbial inactivation and induced structural modification for forming biofilms. New structures are created from aggregates of extracellular polysaccharides and dead bacterial cells, forming a protective and resilient matrix in which the remaining living cells grow and reproduce under proper growth conditions. The new colonies are found to be more resilient in this state, reducing the efficacy of subsequent plasma treatment. We verify that the observed effect is not caused by chemicals produced by plasma reactive species, but instead by the physical processes of drying and convection caused by the plasma discharge. Wiley-VCH Verlag 2018-06-10 Article PeerReviewed Kwandou, Goldina, Mai-Prochnow, Anne, Prescott, Stuart W., Spicer, Patrick T. and Cullen, P.J. (2018) Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms. Plasma Processes and Polymers . ISSN 1612-8869 Atmospheric plasma; Biofilms; Cell agglomeration https://onlinelibrary.wiley.com/doi/10.1002/ppap.201700212 doi:10.1002/ppap.201700212 doi:10.1002/ppap.201700212
spellingShingle Atmospheric plasma; Biofilms; Cell agglomeration
Kwandou, Goldina
Mai-Prochnow, Anne
Prescott, Stuart W.
Spicer, Patrick T.
Cullen, P.J.
Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms
title Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms
title_full Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms
title_fullStr Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms
title_full_unstemmed Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms
title_short Atmospheric air plasma induces increased cell aggregation during the formation of Escherichia coli biofilms
title_sort atmospheric air plasma induces increased cell aggregation during the formation of escherichia coli biofilms
topic Atmospheric plasma; Biofilms; Cell agglomeration
url https://eprints.nottingham.ac.uk/52768/
https://eprints.nottingham.ac.uk/52768/
https://eprints.nottingham.ac.uk/52768/