Real-time ligand binding of fluorescent VEGF-A isoforms that discriminate between VEGFR2 and NRP1 in living cells

Fluorescent VEGF-A isoforms have been evaluated for their ability to discriminate between VEGFR2 and NRP1 in real-time ligand binding studies in live cells using BRET. To enable this, single-site (N-terminal cysteine) labelled versions of VEGF165a, VEGF165b and VEGF121a were synthesised. These were...

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Bibliographic Details
Main Authors:	Peach, Chloe J., Kilpatrick, Laura E., Friedman-Ohana, Rachel, Zimmerman, Kris, Robers, Matthew B., Wood, Keith V., Woolard, Jeanette, Hill, Stephen J.
Format:	Article
Published:	Elsevier 2018
Subjects:	VEGFR2; Neuropilin-1; NanoBRET; Ligand binding kinetics; VEGF isoforms; Receptor mechanisms
Online Access:	https://eprints.nottingham.ac.uk/52730/

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https://eprints.nottingham.ac.uk/52730/

Real-time ligand binding of fluorescent VEGF-A isoforms that discriminate between VEGFR2 and NRP1 in living cells

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